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山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (9): 34-38.doi: 10.6040/j.issn.1671-7554.0.2014.102

• 基础医学 • 上一篇    下一篇

人参皂苷Re对人脐静脉内皮细胞PARP-1表达的抑制作用

陈建华1, 朱邦豪2   

  1. 1. 川北医学院药学院药理教研室, 四川 南充 637000;
    2. 中山大学中山医学院药理教研室, 广东 广州 510000
  • 收稿日期:2014-02-27 修回日期:2014-09-05 出版日期:2014-09-10 发布日期:2014-09-10

Inhibiting Effect of ginsenoside Re on the expression of PARP-1 in human umbilical vein endothelial cell

CHEN Jianhua1, ZHU Banghao2   

  1. 1. Department of Pharmacology, School of Pharmacy, North Sichuan Medical University, Nanchong 637000, Sichuan, China;
    2. Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510000, Guangdong, China
  • Received:2014-02-27 Revised:2014-09-05 Online:2014-09-10 Published:2014-09-10
  • Contact: 朱邦豪。E-mail:zhubh@mail.sysu.edu.cn E-mail:zhubh@mail.sysu.edu.cn

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摘要： 目的 筛选对多聚二磷酸腺苷核糖聚合酶-1（PARP-1）有潜在抑制作用的中药单体，研究中药单体对人脐静脉内皮细胞（HUVEC）PARP-1表达的抑制作用。方法 在中国天然产物数据库中通过计算机虚拟筛选初步得到对PARP-1有潜在抑制作用的6种中药单体，对加入中药单体的细胞分设高、低剂量组，浓度分别为1×10-4、1×10-6 mol/L，另将加入3-氨基苯甲酰胺（3-AB）的细胞作为阳性对照组、只加2×10-4 mol/L ONOO-的细胞作为模型组。Western blotting检测PARP-1表达。若待筛药物对PARP-1表达有抑制作用，则以1×10-4～1×10-8 mol/L的药物进行浓度依赖性实验。结果 1×10-4、1×10-6 mol/L人参皂苷Re与1×10-4 mol/L人参皂苷Rb1对ONOO-引起的PARP-1激活有抑制作用。1×10-4～1×10-8 mol/L人参皂苷Re对 PARP-1 的表达有明显的抑制作用，且呈现出剂量依赖性（P<0.05）。结论 人参皂甙Re对HUVEC上ONOO-激活的PARP-1蛋白表达有抑制作用，对ONOO-引起的细胞损伤具有保护作用，其效应呈现出剂量依赖性。

关键词: 中药单体, ONOO-, 人脐静脉内皮细胞, 多聚二磷酸腺苷核糖聚合酶-1

Abstract: Objective To screen herbal monomers with potential inhibition on poly(ADP-ribose)polymerase-1 (PARP-1), and to study their inhibiting effects on PARP-1 expression in human umbilical vein endothelial cell (HUVEC). Methods Six herbal monomers which might have potential inhibiting effect on PARP-1 were screened by computer visual screening in Chinese Natural Product Database. The cells were divided into the following groups:high (1×10-4 mol/L) and low (1×10-6 mol/L) doses groups for the herbal monomers, the 3-aminobenzamide(3-AB) positive control group and model group. Western blotting was used to test the expression of PARP-1. When the tested drugs exhibited inhibiting effects on PARP-1 expression, the drug-dependent experiments would be carried out with the drug concentration being 1×10-4 to 1×10-8 mol/L. Results 1×10-4 and 1×10-6 mol/L ginsenoside Re and 1×10-4 mol/L ginsenoside Rb1 had inhibiting effect on PARP-1 activated by ONOO-. 1×10-4 to 1×10-8 mol/L ginsenoside Re had obvious inhibiting effect on PARP-1 expression in a dose-dependent manner (P<0.05). Conclusion Ginsenoside Re can inhibit protein expression of PARP-1 activated by ONOO- on HUVECs and has protecting effect on ONOO--induced injury in a dose-dependent manner.

Key words: Human umbilical vein endothelial cell, Herbal monomer, Poly(ADP-ribose)polymerase-1, ONOO-

中图分类号: 

  • R965.1
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