平阳霉素联合透明质酸钠对人淋巴管畸形内皮细胞的作用

doi:10.6040/j.issn.1671-7554.0.2014.098

山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (1): 41-46.doi: 10.6040/j.issn.1671-7554.0.2014.098

平阳霉素联合透明质酸钠对人淋巴管畸形内皮细胞的作用

赵仪云¹, 黄敬春¹, 王月莲¹, 邵山¹, 孙广平¹, 崔恩美¹, 卓绍杨², 刘少华³

1. 济南市第三人民医院口腔科, 山东济南 250101;
2. 山东大学口腔医院口腔颌面外科, 山东济南 250012;
3. 山东大学齐鲁医院口腔科, 山东济南 250012

收稿日期:2014-02-26 修回日期:2014-12-17 发布日期:2015-01-10
通讯作者: 赵仪云。E-mail:y444y@souhu.com E-mail:y444y@souhu.com
基金资助:
国家自然科学基金(81172572);山东省自然科学基金(ZR2010HM008);济南市科技发展计划[2011]34号

Effects of pingyangmycin combined with sodium hyaluronate on human lymphatic malformation endothelial cells

ZHAO Yiyun¹, HUANG Jingchun¹, WANG Yuelian¹, SHAO Shan¹, SUN Guangping¹, CUI Enmei¹, ZHUO Shaoyang², LIU Shaohua³

1. Department of Stomatology, Third Hospital of Jinan, Jinan 250101, Shandong, China;
2. Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Shandong University, Jinan 250012, Shandong, China;
3. Department of Stomatology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China

Received:2014-02-26 Revised:2014-12-17 Published:2015-01-10

摘要/Abstract

摘要： 目的探讨平阳霉素(PYM)联合透明质酸钠(HA)对体外培养的人淋巴管畸形内皮细胞(HLMECs)的作用。方法对新鲜婴幼儿淋巴管畸形组织进行HLMECs原代培养。利用淋巴管内皮特异性标志物LYVE1和PROX1染色,将不同浓度(1、10、100、500 μg/mL)的PYM和(50、100、300、500 μg/mL)HA分别作用于HLMECs 12、24、48 h,观察对HLMECs增殖的影响并筛选其最适有效浓度。然后用该有效浓度的PYM和HA共同作用于HLMECs,在第24小时时,MTT法检测细胞生长情况,TUNEL法检测细胞凋亡情况。结果 ①淋巴管畸形组织和HLMECs表达LYVE1和PROX1;②PYM的最适浓度为10 μg/mL,HA的最适浓度为100 μg/mL;③PYM联合HA在抑制细胞增殖和促进细胞凋亡方面比单纯的PYM作用强。结论 PYM联合HA有望在临床上用于治疗淋巴管畸形。

关键词: 平阳霉素, 淋巴管畸形内皮细胞, 透明质酸钠

Abstract: Objective To explore the effects of pingyangmycin (PYM) combined with sodium hyaluronate (HA) on human lymphatic malformations endothelial cells (HLMECs) in vitro. Methods Primary HLMECs were isolated from a HLMs tissue and cultured with endothelial cell medium (ECM). By lymphatic endothelial specific marker LYVE1 and PROX1 staining, the HLMs specimen and HLMECs were identified. The proliferation effects of HLMECs treated with gradient densities of PYM (1, 10, 100, 500 μg/mL) or HA (50, 100, 300, 500 μg/mL) for 12, 24 and 48 hours, were compared with MTT assay, and the optimal concentration of PYM and HA were selected. After that, the HLMECs were treated with PYM and HA of the optimal concentration. After 24 hours, the cell proliferation and apoptosis were evaluated with MTT assay and TUNEL respectively. Results ① Both LYVE1 and PROX1 were expressed in the HLMs tissue and HLMECs. ② The optimal concentration of PYM and HA was 10 μg/mL and 100 μg/mL respectively. ③ The combination of PYM and HA was more effective than PYM alone on inhibiting HLMECs growth and promoting HLMECs apoptosis. Conclusion The combination of PYM and HA is effective for HLMECs, which can be an alternative in the clinical treatment of lymphatic malformations.

Key words: Lymphatic malformations endothelial cells, Sodium hyaluronate, Pingyangmycin

中图分类号:

R782

赵仪云, 黄敬春, 王月莲, 邵山, 孙广平, 崔恩美, 卓绍杨, 刘少华. 平阳霉素联合透明质酸钠对人淋巴管畸形内皮细胞的作用[J]. 山东大学学报（医学版）, 2015, 53(1): 41-46.

ZHAO Yiyun, HUANG Jingchun, WANG Yuelian, SHAO Shan, SUN Guangping, CUI Enmei, ZHUO Shaoyang, LIU Shaohua. Effects of pingyangmycin combined with sodium hyaluronate on human lymphatic malformation endothelial cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(1): 41-46.

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平阳霉素联合透明质酸钠对人淋巴管畸形内皮细胞的作用

Effects of pingyangmycin combined with sodium hyaluronate on human lymphatic malformation endothelial cells

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