罗哌卡因聚乙二醇-聚乳酸微球对切口痛模型大鼠坐骨神经阻滞的时效

doi:10.6040/j.issn.1671-7554.0.2013.724

山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (3): 50-55.doi: 10.6040/j.issn.1671-7554.0.2013.724

罗哌卡因聚乙二醇-聚乳酸微球对切口痛模型大鼠坐骨神经阻滞的时效

黄海真1，胡启雅1，丁超1，郭亚秋1，齐峰1，任杰2，潘晓军1

1．山东大学齐鲁医院麻醉科，山东济南 250012； 2．山东大学附属济南市中心医院麻醉科，山东济南 250013

收稿日期:2013-12-02 出版日期:2014-03-10 发布日期:2014-03-10
通讯作者: 齐峰。E-mail:qifeng66321@sina.com 任杰。E-mail:jijie-ren@sohu.com
基金资助:
山东大学自主科技创新基金（2012JC017）; 山东省自然科学基金（ZR2012HM097）

Long-term effect of sciatic nerve block with ropivacaine PEG-PLA microparticles in a rat model of postoperative pain

HUANG Haizhen1, HU Qiya1, DING Chao1, GUO Yaqiu1, QI Feng1, REN Jie2, PAN Xiaojun1

1. Department of Anesthesiology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
2. Department of Anesthesiology, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China

Received:2013-12-02 Online:2014-03-10 Published:2014-03-10

摘要/Abstract

摘要：

目的探讨罗哌卡因聚乙二醇–聚乳酸（Rop-PEG-PLA）微球对切口痛模型大鼠坐骨神经阻滞的长期作用。方法 80只雄性Wistar大鼠随机分为4组（每组20只），分别于坐骨神经周围间隙给予0.9% NaCl溶液0.2mL（对照组），PEG-PLA微球10mg（空白微球组），0.5%罗哌卡因0.2mL(含罗哌卡因1mg，罗哌卡因组）或Rop-PEG-PLA微球10mg（含罗哌卡因1mg，罗哌卡因微球组），之后均按照Brennan法制作大鼠切口痛模型。观察术前24h和术后2h、4h、8h、1d、2d、3d、5d和7d时大鼠行为学的变化，免疫组织化学方法检测术后2h和2d时脊髓背角c-fos的表达，HPLC法检测大鼠血浆中罗哌卡因的浓度。结果对照组行为学及c-fos表达与空白微球组的差异无统计学意义（P＞0.05）。术后2h时，罗哌卡因组、罗哌卡因微球组行为学阈值与对照组相比均增高（P＜0.05），c-fos表达均减少（P＜0.05）。罗哌卡因微球组与罗哌卡因组相比，其行为学阈值增高时间（3d vs 2h）及测得血浆药物时间（8h vs 4h）均延长，术后2d时c-fos表达减少（P＜0.05）。结论 Rop-PEG-PLA微球可延长切口痛模型大鼠坐骨神经阻滞镇痛时间达3d。

关键词: 聚乳酸；微球；坐骨神经阻滞；c-fos, 罗哌卡因；聚乙二醇&ndash

Abstract:

Objective To investigate the efficiency of ropivacaine PEG-PLA microspheres for sciatic nerve block in rats. Methods Eighty Wistar rats were randomly divided into four groups (n=20, each) and were treated with 0.2mL of 0.9% saline(control group),10mg of PEG-PLA(PEG-PLA group), 0.2mL of 0.5% ropivacaine(ropivacaine group) and 10mg of 10% Rop-PEG-PLA microspheres(Rop-PEG-PLA group), respectively. The incision pain models were produced as described by Brennan. Mechanical thresholds and thermal latencies were measured at 24h before incision and at 2, 4, 8h, as well as 1, 2, 3, 5 and 7d after incision. Expression of c-fos protein in the spinal cord was determined by immunohistochemistry at 2h and 2d after incision. The ropivacaine concentration in plasma of ropivacaine group and Rop-PEG-PLA group was detected by HPLC analysis. Results Behavioral testing and c-fos expression in PEG-PLA group showed no significant difference compared with those in control group(P＞0.05). The thresholds and latencies in ropivacaine group and Rop-PEG-PLA group were significantly higher than those in control group at 2h after incision(P＜0.05). Expressions of c-fos protein were suppressed in ropivacaine group and Rop-PEG-PLA group at 2h after incision, compared with that in control group(P＜0.05). The duration of anti-hyperalgesia effect（3d vs 2h）and ropivacaine concentration in plasma ( 8h vs 4h) in Rop-PEG-PLA group were longer than those in ropivacaine group, and c-fos expression in Rop-PEG-PLA group was lower than that in ropivacaine group at 2d after incision (P＜0.05). Conclusion Single administration of ropivacaine PEG-PLA microspheres for sciatic nerve block provides effective analgesia for postoperative pain for 3d.

Key words: Microparticle, Ropivacaine, Sciatic nerve block, c-fos, Poly (ethylene glycol)-co-poly (D,L-lactic acid)

中图分类号:

R614

黄海真1，胡启雅1，丁超1，郭亚秋1，齐峰1，任杰2，潘晓军1. 罗哌卡因聚乙二醇-聚乳酸微球对切口痛模型大鼠坐骨神经阻滞的时效[J]. 山东大学学报（医学版）, 2014, 52(3): 50-55.

HUANG Haizhen1, HU Qiya1, DING Chao1, GUO Yaqiu1, QI Feng1, REN Jie2, PAN Xiaojun1. Long-term effect of sciatic nerve block with ropivacaine PEG-PLA microparticles in a rat model of postoperative pain[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(3): 50-55.

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罗哌卡因聚乙二醇-聚乳酸微球对切口痛模型大鼠坐骨神经阻滞的时效

Long-term effect of sciatic nerve block with ropivacaine PEG-PLA microparticles in a rat model of postoperative pain

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