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山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (11): 106-112.doi: 10.6040/j.issn.1671-7554.0.2014.449

• 公共卫生与管理学 • 上一篇    

脂联素基因和钙蛋白酶10基因多态性与结直肠癌发生风险的关系

刘文辉1, 李晓玲1, 袁萍1, 栾荣生1, 冯斐1, 胡晓琴1, 燕锦2, 杨艳芳1   

  1. 1. 四川大学华西公共卫生学院流行病与卫生统计学系, 四川 成都 610041;
    2. 四川省肿瘤医院肠道外科, 四川 成都 610041
  • 收稿日期:2014-07-08 发布日期:2014-11-10
  • 通讯作者: 杨艳芳.E-mail:yang2009@scu.edu.cn E-mail:yang2009@scu.edu.cn
  • 基金资助:
    国家自然科学基金(81102196);教育部博士点专项科研基金(20090181120019)

Genetic polymorphisms of ADIPOQ and CAPN-10 and colorectal cancer risk

LIU Wenhui1, LI Xiaoling1, YUAN Ping1, LUAN Rongsheng1, FENG Fei1, HU Xiaoqin1, YAN Jin2, YANG Yanfang1   

  1. 1. Department of Epidemiology and Biostatistics, West China School of Public Health, Sichuan University, Chengdu 610041, Sichuan, China;
    2. Department of Intestinal Surgery, Sichuan Cancer Hospital, Chengdu 610041, Sichuan, China
  • Received:2014-07-08 Published:2014-11-10

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摘要： 目的 探讨脂联素基因(ADIPOQ)rs2241766和rs1501299、钙蛋白酶10基因(CAPN-10)rs3842570和rs3792267多态性以及基因之间的交互作用与结直肠癌发生风险的关系.方法 采用1:1匹配的病例对照研究,对400例经组织病理学确诊的结直肠癌原发、新发病例和400例同期未患结直肠癌的健康体检者进行问卷调查,收集两组研究对象的一般情况、生活方式及饮食习惯等资料.应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析法检测研究对象ADIPOQ(rs2241766和rs1501299)和CAPN-10(rs3842570和rs3792267)的基因型.采用条件logistic回归模型分析4个位点的基因多态性与结直肠癌发生风险的关系,并应用GMDR法分析4个位点间的交互作用.结果 ADIPOQ rs2241766 TG+GG基因型携带者结直肠癌发生风险较TT型携带者高(OR=1.354,95%CI:1.004～1.827),rs1501299 GT+TT基因型携带者结直肠癌发生风险较GG型携带者低(OR=0.680,95%CI:0.501～0.923);CAPN-10 rs3792267 GA+AA基因型携带者较GG型携带者结直肠癌发生风险高(OR=1.507,95%CI:1.009～2.250),未见 rs3842570 Del/Ins+Ins/Ins基因型与结直肠癌的发生风险有关(OR=1.345,95%CI:0.849～2.130).GMDR法分析结果显示,4个位点在结直肠癌的发生中可能存在交互作用(P=0.001).趋势χ2分析结果表明,风险基因型携带个数与结直肠癌发生风险之间存在剂量-反应关系(χ2=13.302,P<0.001).结论 ADIPOQ(rs2241766和rs1501299)和CAPN-10 rs3792267 多态性可能与结直肠癌的发生风险有关,4个位点间可能存在交互作用,共同影响结直肠癌的发生.

关键词: 基因多态性, 钙蛋白酶10基因, 交互作用, 结直肠癌, 脂联素基因

Abstract: Objective To explore the associations of ADIPOQ (rs2241766, rs1501299) and CAPN-10 (rs3842570, rs3792267) polymorphisms and gene-gene interaction with the risk of colorectal cancer(CRC). Methods A 1:1 matched case-control study was conducted to collect the demographic features, lifestyle, and dietary habits of 400 patients with newly histopathologically diagnosed CRC and 400 matched health controls. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the genotypes of ADIPOQ (rs2241766, rs1501299) and CAPN-10 (rs3842570, rs3792267). Conditional logistic regression was used to analyze the association of genetic polymorphisms of the 4 SNPs and CRC risk. Generalized multifactor dimensionality reduction (GMDR) was applied to test the gene-gene interaction on CRC risk. Results Compared with the wild genotypes, subjects carryingADIPOQ rs2241766 TG+GG genotype and CAPN-10 rs3792267 GA+AA genotype had the increased CRC risk (rs2241766: OR=1.354, 95%CI: 1.004-1.827; rs3792267: OR=1.507, 95%CI: 1.009-2.250), while those carrying ADIPOQ rs1501299 GT+TT genotype had an decreased CRC risk (OR=0.680, 95%CI:0.501-0.923). The association between CAPN-10 rs3842570 Del/Ins+Ins/Ins genotype and CRC risk was not significant (OR=1.345, 95%CI: 0.849-2.130). The GMDR showed that the gene-gene interaction among the 4 locus on CRC risk was significant (P=0.001). A significant dosage effect with an increasing number of risk genotypes was observed as the risk of CRC increased (χ2=13.302, P<0.001). Conclusion ADIPOQ rs2241766, ADIPOQ rs1501299, and CAPN-10 rs3792267 are associated with the risk of CRC, and the 4 locus might affect the risk of CRC together.

Key words: Genetic polymorphisms, Calpain 10 gene, Interactions, Colorectal cancer, Adiponectin gene

中图分类号: 

  • R735.3
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