重组人生长激素对烧伤大鼠肠粘膜细胞凋亡的作用

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (5): 59-62.

重组人生长激素对烧伤大鼠肠粘膜细胞凋亡的作用

石文辛乃军马印东杨涛贾军姜金珠郭成浩宋国栋

山东大学 1. 附属济南市中心医院烧伤整形科，济南 250013； 2. 医学院病理学与病理生理学研究所，济南 250012

收稿日期:2008-11-30 出版日期:2009-06-16 发布日期:2009-05-16
通讯作者: 宋国栋（1962- ），男，博士，主任医师，主要从事肠源性感染、创伤愈合及组织工程研究。
作者简介:石文（1983- ），女，硕士研究生，主要从事肠源性感染防治研究。
基金资助:
济南市科技发展计划项目（021035）。

Effect of recombinant human growth hormone on intestinal mucosal cellular apoptosis in severely scalded rats

SHI Wen, XIN Nai-Jun, MA Yin-Dong, YANG Tao, JIA Jun, JIANG Jin-Zhu, GUO Cheng-Hao, SONG Guo-Dong

1. Department of Burn and Plastic Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China; 2. Institute of Pathology and Pathophysiology, School of Medicine, Shandong University, Jinan 250012, China

Received:2008-11-30 Online:2009-06-16 Published:2009-05-16

摘要/Abstract

摘要：

目的观察早期应用重组人生长激素（rhGH）对严重烧伤大鼠小肠粘膜上皮细胞凋亡的作用并探讨其可能的作用机制。方法 42只成年雄性Wistar大鼠随机分为对照组（n=6只）、烧伤组（n=18只）和GH组（n=18只）。后两组造成25%总体表面积Ⅲ度烫伤，立即腹腔注射地塞米松80mg/kg，从伤后2h开始连续3d分别皮下注射等渗盐水和rhGH［1.33U/（kg·d）］。于伤后12、24和72h，HE染色观察末端回肠粘膜组织形态，TUNEL法检测小肠粘膜上皮细胞凋亡形态及凋亡率，荧光法检测肠粘膜细胞Caspase-3酶活性变化。结果烧伤组肠粘膜形态结构及上皮细胞损伤严重，GH组明显减轻，基本接近对照组。在伤后各时相点，烧伤组及GH组肠粘膜上皮细胞凋亡率均较对照组显著增加(P<0.01)，但GH组显著低于烧伤组（P<0.01）,两组均于伤后24h达高峰［（15.40±3.21）% vs （38.80±6.98）%］；烧伤组及GH组Caspase-3酶活性亦明显高于对照组（P<0.05），GH组明显低于烧伤组（P<0.05），亦于伤后24h达高峰［（156.13±4.66）U/mg vs （214.60±9.29）U/mg］。结论早期应用rhGH能明显抑制严重烧伤大鼠肠粘膜上皮细胞凋亡，可能通过下调Caspase-3酶活性抑制肠粘膜细胞凋亡，减轻肠粘膜损伤，维护其形态结构与屏障功能。

关键词: 烧伤, 肠粘膜屏障, 生长激素(人), 细胞凋亡

Abstract:

Abstract: Objective To investigate whether recombinant human growth hormone (rhGH) can improve the intestinal mucosal barrier in severely scalded rats and by which possible mechanisms these changes occur. Methods 42 adult male Wistar rats were randomly divided into 3 groups: the control（n=6）, scald(n=18) and GH groups(n=18). Rats in the scald and GH groups were inflicted with 25% total body surface area full-thickness skin scald on the back and immediately followed by intraperitoneal injection of dexamethasone (80mg/kg). The scalded rats in the two groups were subcutaneously injected with normal saline and rhGH ［1.33U/（kg·d）］ respectively from 2 hours to 3 days after scald. At 12, 24 and 72h after scald, the intestinal mucosal histomorphology in distal ileum tissues, morphological changes of apoptotic cells and apoptotic rate and changes of activity of Caspase-3of intestinal mucosal cells were observed by HE stain, TUNEL and fluorescence method, respectively. Results Intestinal mucosal histomorphology and intestinal epithelia in the scald group was severely injured but significantly melioratedby rhGH to near that in the control group. The intestinal mucosal cellular apoptotic rate in both scald and GH groups was significantly higher than that in thecontrol group(P<0.01) while that in the GH group was evidently lower than that in the scald group (P<0.01),and reached a peak （15.40%±3.21% vs 38.80%±6.98%） at 24h after burn. Also, activity of Caspase-3 was more significantly increased in the scald and GH groups than in the control group(P<0.05) while it was lower in the GH group than in the scald group(P<0.05), and reached a peak （156.13±4.66U/mg vs 214.60±9.29U/mg） at 24h. Conclusion rhGH administered inearly severe burn could inhibit intestinal mucosal cellular apoptosis, which might be induced by down-regulation of activity of Caspase-3. As a result, intestinal mucosal integrity and function was maintained.

Key words: Burns; Gut barrier; Somatropin; Apoptosis

中图分类号:

R644

石文辛乃军马印东杨涛贾军姜金珠郭成浩宋国栋. 重组人生长激素对烧伤大鼠肠粘膜细胞凋亡的作用[J]. 山东大学学报(医学版), 2009, 47(5): 59-62.

SHI Wen, XIN Nai-Jun, MA Yin-Dong, YANG Tao, JIA Jun, JIANG Jin-Zhu, GUO Cheng-Hao, SONG Guo-Dong. Effect of recombinant human growth hormone on intestinal mucosal cellular apoptosis in severely scalded rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(5): 59-62.

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