关键词: 鱼藤酮, 前列腺凋亡反应蛋白-4, 细胞凋亡, PC12细胞, 信号传导

Abstract:

Objective  To study the prostate apoptosis response-4 (Par-4) expression in PC12 cells after retonone treatment and to provide a new target for the treatment of Parkinson′s disease (PD).  Methods: 1, 5, or 10μmol/L rotenone was given to PC12 cells for 6h and 24h. MTT assay was used for determining the viability rate, and western blot was used to determine the protein level of Par-4.  Results  After rotenone treatment, cells showed different toxic appearances. After the 6h-treatment, the photodensity ratio of Par-4 to actin in the 10μmol/L group（P＝0.029 646）was significantly increased compared with the normal control group. After being pre-treated with 20μmol/L Ginestin, an inhibitor of the JNK pathway, for 1 hour, the ratio was decreased. However in the24h groups, the viability rates significantly decreased, and ratios in the 1, 5μmol/L (P=0.018461, P=0.043415) groups were increased compared with the 10μmol/L group（P=0.081 298）.  Conclusion Expression of Par-4 in PC12 cells is increased after rotenone treatment in a time and concentrationdependent manner. The JNK pathway participates in the process of Par-4 stepping up.

Key words: Rotenone; Prostate apoptosis response-4; Apoptosis;PC12 cell; Signal transduction