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山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (3): 70-74.doi: 10.6040/j.issn.1671-7554.0.2016.1186

• 基础医学 • 上一篇    下一篇

贵州省2013年麻疹病毒核蛋白基因特征分析

唐小敏1,张丽1,叶绪芳1,任刚1,左丽2   

  1. 1.贵州省疾病预防控制中心免疫规划所, 贵州 贵阳 550004;2.贵州医科大学基础医学院免疫学教研室, 贵州 贵阳 550025
  • 收稿日期:2016-09-20 出版日期:2017-03-10 发布日期:2017-03-10
  • 通讯作者: 左丽. E-mail:zuoligymc@163.com E-mail:zuoligymc@163.com

Nucleoprotein gene analysis of measles virus in Guizhou Province in 2013

TANG Xiaomin1, ZHANG Li1, YE Xufang1, REN Gang1, ZUO Li2   

  1. 1. Department of Immunization Programs, Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, Guizhou, China;
    2. Department of Immunology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, China
  • Received:2016-09-20 Online:2017-03-10 Published:2017-03-10

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摘要： 目的 分析2013年贵州省流行的麻疹病毒核蛋白基因特征。 方法 采集疑似麻疹病例临床标本(咽拭子、尿液和血清),从临床标本或Vero/SLAM细胞分离出的阳性培养物中提取病毒RNA,采用RT-PCR方法扩增麻疹病毒核蛋白基因羧基末端编码的634个核苷酸片段,并进行序列测定与基因特征分析。 结果 74例疑似麻疹病例中检测到41株麻疹核酸。基因亲缘关系显示39株为H1基因型中的H1a基因亚型并且有2个明显分支;2株为麻疹疫苗株(A基因型)。39株麻疹病毒株与H1基因型参考株MVi/Hunan.CHN/0.93/7(AF045212)的核苷酸和氨基酸差异分别为2.0%~2.9%和2.0%~4.7%;与H1a基因亚型参考株Chin9322的核苷酸和氨基酸差异分别为0.9%~1.8%和0.7%~3.4%;39株H1a基因亚型的核苷酸和氨基酸同源性分别为97.3%~100%和96.6%~100%。2株麻疹疫苗株与MV沪191(S191)的核苷酸和氨基酸同源性分别为99.8%~100%和99.3%~100%。 结论 H1a基因亚型是贵州省2013年本土麻疹流行的优势基因型,同一年份存在着不同的传播链。

关键词: 基因型, 核蛋白, 麻疹病毒, 序列分析

Abstract: Objective To analyze the nucleoprotein genes of measles virus in Guizhou Province in 2013. Methods The throat swab, urine and sera samples were collected. Virus RNA was extracted directly from clinical specimens or cultured positive product of Vero/SLAM cell separation. Fragments of 634 nucleotide acids at the carboxyl end of the nucleoprotein gene were amplified by RT-PCR and then the PCR products were sequenced and analyzed. Results A total of 41 strains of measles virus were detected from 74 suspected measles cases. The results of phylogenetic analysis showed that 39 strains of measles virus were identified as H1a sub-genotype of H1 genotype, and located in two obvious branches. Two strains of them were identified as measles vaccine strain(A genotype). The nucleotide and amino acid divergences between 39 strains of measles virus strains and H1 genotype reference strain MVi/Hunan CHN/0.93/7(AF045212)were 2.0%-2.9% and 2.0%-4.7%, respectively. The nucleotide and amino acid divergences between 39 strains of measles virus strains and WHO H1a sub-genotype reference strain(Chin9322)were 0.9%-1.8% and 0.7%-3.4%, respectively. In addition, the nucleotide and amino acid homology among 39 MV strains of H1a sub-genotype were 97.3%-100% and 96.6%-100%, respectively. The nucleotide and amino acid homology between 2 strains of measles vaccine strains and S191 were 99.8%-100% and 99.3%-100%, respectively. Conclusion H1a sub-genotype was the predominant genotype in Guizhou Province in 2013, and there existed different chain of transmission in one year.

Key words: Genotype, Sequence analysis, Nucleoprotein, Measles virus

中图分类号: 

  • R373.11
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