局部应用辛伐他汀促进前交叉韧带重建骨隧道腱骨愈合的效应

doi:10.6040/j.issn.1671-7554.0.2014.887

摘要/Abstract

摘要： 目的探讨局部应用辛伐他汀促进腱骨愈合的效应。方法采用15只大白兔建立实验模型, 取自体趾长屈肌腱进行前交叉韧带(ACL)重建, 胫骨侧应用螺钉固定, 股骨侧采用悬吊固定。左侧作为实验侧, 在股骨隧道远关节侧填塞入辛伐他汀明胶海绵复合物;右侧为对照侧, 填塞入明胶海绵。术后2、4、8周各处死3只做组织学检查, 术后8周处死6只做生物力学检查, 观察腱骨愈合情况, 通过对比判断辛伐他汀促进腱骨愈合的效应。结果实验侧术后2周腱骨界面见大量新生血管, 结合紧密;4周可见大量sharpey纤维形成;8周腱骨界面模糊, 可见分层软骨。对照侧术后4周未见sharpey纤维形成, 8周见sharpey纤维形成。生物力学检测发现, 术后8周实验侧股骨隧道拉脱负荷高于对照侧, 差异有统计学意义(P<0.05)。结论局部应用辛伐他汀具有促进ACL重建骨隧道腱骨愈合的作用。

关键词: 腱骨愈合, 前交叉韧带, 重建, 辛伐他汀

Abstract: Objective To evaluate the enhancement potential of topical use of simvastatin for tendon-bone healing. Methods A total of 15 rabbits were enrolled to establish anterior cruciate ligament (ACL) reconstruction models. The tibial side was screw fixed while the femoral side was suspending fixed in both knees of the rabbits. Simvastatin was inserted into the left knee (experimental side) bone tunnel with a gelatin sponge carrier, while gelatin sponge was inserted into the right knee (control side) bone tunnel. Three rabbits were sacrificed in week 2, 4 and 8 postoperatively. Tendon-bone interface was histologically assessed. Six rabbits were sacrificed for biomechanical tests in week 8. Results Histological analysis in week 2 demonstrated that tendon-bone healing was significantly greater with angiogenesis in the simvastatin-treated side than in the control side. In week 4, a great quantity of sharpey fibers at tendon-bone interface were observed in the experimental side, yet there were no sharpey fibers in the control side. In week 8, tendon-bone interface was narrow and unclear in the experimental side, yet a few sharpey fibers were observed in the control side. The average load to failure in the experimental side was significantly greater than in the control side. Conclusion Topical use of simvastatin promotes the tendon-bone healing after ACL reconstruction.

Key words: Reconstruction, Simvastatin, Tendon-bone healing, Anterior cruciate ligament

中图分类号:

R686.5

林锦秀, 孙东升, 郑潇, 尹德超, 李晔, 张磊. 局部应用辛伐他汀促进前交叉韧带重建骨隧道腱骨愈合的效应[J]. 山东大学学报（医学版）, 2015, 53(3): 27-31.

LIN Jinxiu, SUN Dongsheng, ZHENG Xiao, YIN Dechao, LI Ye, ZHANG Lei. Enhancement of tendon-bone healing by topical use of simvastatin after ACL reconstruction[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(3): 27-31.

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