关键词: 肠黏膜, 三叶因子3, 烧伤, 小鼠

Abstract:

Objective   To explore the effect of recombinant hTFF3 on burn-induced intestinal injury in mice. Methods  Kunming mice were inflicted on the back with III degree burn injury on 30 % of total body surface, and then they were randomly divided into three experimental groups: sham + saline group,  burn + saline group, and burn + hTFF3 (group TFF3). Mice in group TFF3 were exposed to thermal injury, administered orally with hTFF3 protein (1mg/kg) dissolved in saline by gastric gavage immediately after recovering from anesthesia, and continued with the same dose once a day. Mice in  burn + saline group received thermal injury and saline in equal volume once a day. Mice in  sham + saline group were exposed to 21℃ water and saline after recovering from anesthesia. Pathomorphological changes, villous height, crypt depth, and mortality rate after injury were observed. Results  Gastric gavage with hTFF3 (1mg/kg) daily significantly alleviated the damage and pathological changes of intestinal mucosa. Mice in  burn + saline group manifested hemorrhage, necrosis and ulcer. Mice in group hTFF3 presented congestion and edema, and at the same time, the thickness of intestinal mucosa, the height of villous and depth of crypt were markedly increased, and the death rate at day 5 was reduced(27.3% vs 45.6%).  Conclusion  Recombinant hTFF3 has significant therapeutic effect on the burn-induced intestinal injury by promoting the reconstruction of injured intestinal mucosa.

Key words: Trefoil factor 3, Burn, Intestinal mucosa； Mice