烟曲霉菌提取物对人支气管上皮细胞修复功能的影响

doi:10.6040/j.issn.1671-7554.0.2014.506

摘要/Abstract

摘要： 目的探讨烟曲霉菌对呼吸道上皮细胞修复功能的影响及其可能机制.方法实验分为正常对照组、不同浓度(10、20、40 mg/L)AFE组、热处理AFE组.分别应用不同浓度和热处理的烟曲霉菌提取物(aspergillus fumigatus extract,AFE)作用体外培养的人支气管上皮细胞16HBE不同的时间,以细胞体外损伤修复功能、细胞迁移能力、定量细胞黏附能力、细胞伸展面积为指标,观察AFE和热处理的AFE对上皮细胞修复能力的影响.结果 20、40 mg/L AFE均明显抑制16HBE细胞的体外损伤修复功能、细胞迁移能力和细胞黏附能力(P<0.05),并呈明显的时间和浓度依赖性.10 mg/L AFE与正常对照组差异无统计学意义(P>0.05).10、20、40 mg/L AFE均明显抑制细胞的伸展能力(P<0.05),并呈明显浓度依赖性.另外,热处理AFE的蛋白酶完全灭活,对上述观察指标无明显影响(P>0.05).结论 AFE依赖其蛋白酶活性明显抑制细胞的修复功能,并呈明显的浓度和时间依赖性.

关键词: 烟曲霉菌, 修复功能, 蛋白酶, 支气管上皮细胞

Abstract: Objective To investigate the effects of aspergillus fumigatus extract (AFE) on the human bronchial epithelial cells repair ability and its possible mechanism. Methods The cells were divided into medium control group, different concentrations (10, 20 and 40 mg/L) of AFE groups and heat-treated AFE group. Human bronchial epithelial cells 16HBE were cultured in vitro, which were exposed to different concentrations of AFE for different time. Wound closure assay, cell migration, quantitative cell attachment assay and spreading of bronchial epithelial cells were observed. AFE and heat-treated AFE were used to investigate the effects of AFE on the human bronchial epithelial cells repair ability. Results Compared with medium control group, the repair capacity, migration ability and adhesion ability of 16HBE cells were inhibited significantly in 20 and 40 mg/L groups (P<0.05) with a time- and concentration-dependent manner; however, there was no obvious change in 10 mg/L AFE group (P>0.05). The spreading ability of 16HBE cells in 10, 20 and 40 mg/L AFE groups were inhibited with a concentration-dependent manner (P<0.05). Heat-treated AFE, whose protease activities were lost, showed no effects on the indicators mentioned above (P<0.05). Conclusion AFE, depending on its protease activity, can inhibit the repair ability of human bronchial epithelial cells, which is positively related to the exposure time and concentration of AFE.

Key words: Repair ability, Aspergillus fumigatus, Bronchial epithelial cell, Protease

中图分类号:

R363

龙飞, 苏莉莉, 梁斌, 金先桥. 烟曲霉菌提取物对人支气管上皮细胞修复功能的影响[J]. 山东大学学报（医学版）, 2014, 52(11): 21-26.

LONG Fei, SU Lili, LIANG Bin, JIN Xianqiao. Effects of Aspergillus fumigatus extract on human bronchial epithelial cells repair ability[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(11): 21-26.

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