关键词: 骨髓祖代细胞, 一氧化碳中毒, 移植, 细胞凋亡

Abstract:

 Objective  To investigate the effect and mechanism of mesenchymal stem cells (MSCs) transplanted for brain damage induced by carbon monoxide poisoning (COP) in rats.  Methods  MSCs were isolated from the bone marrow of Wistar rats, cultured and generated in vitro and then made into a suspension. The rat carbon monoxide poisoning models were established by peritoneal injection of 100-150mL/kg CO. MSCs marked with Bromodeoxyuridine(Brdu) were transferred into rats through the internal carotid artery. The Morris water labyrinth experiment was used to examine the nervous system function of the model rats. Damage area cells in the cornu ammonis were determined with an immunohistochemical method and Nissl'sstaining.  Results   MSCs can survive and reduce apoptosis of nerve cells. One and four weeks later, Morris water labyrinth experiments demonstrated that the rats′ space learning capability greatly improved and pathological changes of the brain organization were obviously reduced(P＜0.05).   Conclusion   Mesenchymal stem cells (MSCs) transplantation is effect in treating brain damage induced by carbon monoxide poisoning (COP) in rats.

Key words: Myeloid progenitor cells; Carbon monoxide poisoning； Transplantation; Apoptosis