关键词: 异基因外周造血干细胞移植, 贫血，再生障碍性, 儿童

Abstract:

To investigate the effects of allogeneic peripheral blood stem cell transplantation (allo—PBSCT) for severe aplastic anemia(SAA) in children. MethodsEight child patients, six boys and two girls， with a median age of 11 years (range：714) with SAA (four patients with SAAI and four with SAAⅡ) received allo—PBSCT of related donors． Most in this group had been treated with CSA and other treatments which proved to be ineffective before transplantation． The period from diagnosis to transplantation was 145 months， with a median time of 13 months． Being conditioned with a decreased dosage of immunosuppressive agents of CTX and antilymphocyte globulin(ALG) or antithymocyte globulin(ATG), patients were given CsA and MTX to prevent graftversushost disease (GVHD)．The engraftment was identified by means of  STRPCR. ResultsAll acquired fast reconstruction was with full donor cell grafting. The median periods for neutrophil ≥0.5×109／L and platelet ≥20×109／L were 12.75 (1015)and 19.88(1532) days， respectively. One patient had a decreased pancytopenia 10 months after transplantation； STRPCR indicated graft rejection. This patient was treated with ATG and CsA,  and then hematopoiesis of the patient gradually recovered. One patient developed gradeⅡ aGVHD and one developed limited cGVHD．All the patients survived with a median period of 29 months (15 50) at the end of followup. ConclusionAlloPBSCT with a conditioning regimen of ATG plus CTX is an effective therapy with few complications for child patients with SAA and is worth popularizing in clinical practice．

Key words: Allogeneic peripheral blood stem cells transplantation, Anemia, aplastic, Children