趋化因子CXCL10与肿瘤细胞RNA修饰树突状细胞抗前列腺癌研究

山东大学学报(医学版)

趋化因子CXCL10与肿瘤细胞RNA修饰
树突状细胞抗前列腺癌研究

李博¹,赵庆利¹,伦晓勤²,魏学斌¹,李顺¹,李青¹

(1. 山东大学附属千佛山医院泌尿外科，济南 250014； 2. 济南医院职业病科，济南 250014)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-02-16 发布日期:2009-02-16

Immunotherapeutic effect of dendritic cell vaccine modified with the CXCL10
gene and tumor cell RNA on mice with prostate cancer

LI Bo¹, ZHAO Qingli¹, LUN Xiaoqin², WEI Xuebin¹, LI Shun¹, LI Qing¹

(1. Department of Urology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China;2. Department of Occupational Diseases, Jinan Hospital, Jinan 250014, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-02-16 Published:2009-02-16

摘要/Abstract

摘要： 目的探讨肿瘤细胞RNA和趋化因子CXCL10基因共同修饰的树突状细胞(DC)瘤苗对小鼠抗肿瘤免疫反应的诱导作用。方法将小鼠RM1前列腺癌细胞RNA加载小鼠骨髓来源的DC后,通过脂质体转染法将CXCL10基因导入DC,制备前列腺癌瘤苗;体内外实验检测该瘤苗诱导的抗前列腺癌免疫治疗作用。结果转染CXCL10基因的DC上清对淋巴细胞有较强的趋化作用;DC瘤苗所诱导的特异性效应细胞(CTL)对RM1细胞具有特异性杀伤作用;经DC瘤苗治疗的荷瘤小鼠模型瘤体生长减慢,存活期延长;该瘤苗还具有明显的免疫保护作用。结论构建的前列腺癌DC瘤苗能够有效诱导抗肿瘤免疫反应和免疫保护功能。

关键词: 树突细胞, RNA,肿瘤, CXCL10基因, 前列腺肿瘤, 小鼠

Abstract: To evaluate the effect of therapeutic vaccine against prostate cancer based on dendritic cell (DC) vaccine modified with tumor cell RNA and the CXCL10 gene both in vitro and in vivo. MethodsMouse bone marrow DC was pulsed with cell RNA from the RM1 prostate cancer cell line and then transfected with the recombinant plasmid CXCL10／pcDNA31 by DOTAP liposome. The immunotherapeutic effects of DC vaccine on mice with prostate cancer were assessed. ResultsThe DC vaccine increased the preferential chemotaxis to T cells, and the mice immunized with DC vaccine exhibited a specific CTL response compared with other groups. In the C57BL／6 mice model with preestablished subcutaneous RM1 prostate cancer, immunization of DC vaccine inhibited tumor growth more significantly when compared with other counterparts and the survival time of micetreated with the DC vaccine was greatly extended. The DC vaccine immunized mice also exhibited an effective resistance to tumor challenge. ConclusionDC vaccine modified with CXCL10 gene and tumor cell RNA might elicit significant antitumor effects through efficient induction of antitumor immunity and might be of therapeutic potentials for prostate cancer.

Key words: Dendritic cells, RNA,neoplasm, CXCL10 Genes, Prostatic neoplasms, Mice

中图分类号:

R73725

李博1 ,赵庆利1 ,伦晓勤2 ,魏学斌1 ,李顺1 ,李青1
. 趋化因子CXCL10与肿瘤细胞RNA修饰
树突状细胞抗前列腺癌研究[J]. 山东大学学报(医学版), 2009, 47(02): 10-13.

LI Bo¹, ZHAO Qingli¹, LUN Xiaoqin², WEI Xuebin¹, LI Shun¹, LI Qing¹

. Immunotherapeutic effect of dendritic cell vaccine modified with the CXCL10
gene and tumor cell RNA on mice with prostate cancer
[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(02): 10-13.

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