乙型肝炎病毒感染与宿主淋巴细胞HLA-Ⅰ类分子关系的研究

山东大学学报(医学版)

乙型肝炎病毒感染与宿主淋巴细胞HLA-Ⅰ类分子关系的研究

邓小梅1，王金凤2，邹雄2，于修平3，杨晓静2，郑桂喜2,刘敏2

山东大学 1．齐鲁医院脑脊液细胞室2．齐鲁医院检验科； 3．医学院病毒研究所，山东济南 250012

收稿日期:2005-09-09 修回日期:1900-01-01 出版日期:2006-12-24 发布日期:2006-12-24
通讯作者: 邓小梅

Relationship between HBV infection and expression ofHLA molecules on host lymphocytes

DENG Xiao-mei1, WANG Jin-feng2, ZOU Xiong2, YU Xiu-ping3, YANG Xiao-jing2, ZHENG Gui-xi2, LIU Min2

1. Laboratory of Cerebrospinal Fluid, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;2. Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;

Received:2005-09-09 Revised:1900-01-01 Online:2006-12-24 Published:2006-12-24
Contact: DENG Xiao-mei

摘要/Abstract

摘要： 目的：检测慢性乙型肝炎、肝硬化及肝癌患者外周血淋巴细胞HLAⅠ、HLAⅡ类分子表达，并探讨其在宿主细胞免疫状态中的作用。方法：采用流式细胞术(FCM)检测51例正常人、58例慢性乙型肝炎、31例肝硬化、13例肝癌患者外周血淋巴细胞表面HLAⅠ、HLAⅡ类抗原的表达。结果：慢性乙型肝炎、肝硬化、肝癌患者外周血淋巴细胞HLAⅠ类分子平均荧光强度分别为335.80、309.78、114.04，均较正常对照组 (565.08) 降低（P<0.01），且随病情加重逐步降低。正常对照、慢性乙型肝炎、肝硬化、肝癌患者HLAⅡ类分子表达率分别为32.50%、38.28%、42.21%、52.75%，其中肝硬化组和肝癌组与正常对照组差异有统计学意义（P<0.01），慢性乙型肝炎组和正常对照组差异无统计学意义（P>0.05）。结论：慢性乙型肝炎患者外周血淋巴细胞HLAⅠ类分子表达降低，肝硬化和肝癌患者降低更为显著，这与患者细胞免疫功能紊乱密切相关。

关键词: 肝硬化, 慢性, 肝炎, 乙型, 肝炎病毒, 乙型, 癌, HLA抗原

Abstract: To examine the expression of HLA class Ⅰ and HLA class Ⅱ on peripheral blood lymphocytes in patients with chronic hepatitis B, hepatic cirrhosis and hepatocellular cancer and investigate the role of them on peripheral blood lymphocytes in host celluar immunity. Methods: The expression of HLA class Ⅰ and HLA class Ⅱ on peripheral blood lymphocytes were evaluated by flow cytometry (FCM) in 51 healthy persons, 58 patients with chronic hepatitis B, 58 patients with hepatic cirrhosis and 13 patients with hepatocellular cancer. Results: The mean fluoresecence intensity of HLA class Ⅰ on peripheral blood lymphocytes in patients with chronic hepatitis B, hepatic cirrhosis and hepatocellular cancer was 335.80, 309.78 and 114.04, respectively, which had a significant difference from that in the normal control(P<0.01). With the aggravation of disease, the mean fluoresecence intensity of HLA class Ⅰ on peripheral blood lymphocytes progressively decreaced in patients with chronic hepatitis B, hepatic cirrhosis and hepatocellular cancer. The expressive percentage of HLA class Ⅱ in patients with chronic hepatitis B, hepatic cirrhosis and hepatocellular cancer was 38.28%, 42.21% and 52.75%, respectively, and there was a significant difference in patients with hepatic cirrhosis and hepatocellular cancer from that in normal controls (P<0.01); but there was

Key words: HLA antigens, Hepatitis B virus, Hepatitis B, chronic, Liver cirrhosis, Carcinoma, hepatocellular

邓小梅,王金凤,邹雄,于修平,杨晓静,郑桂喜,刘敏. 乙型肝炎病毒感染与宿主淋巴细胞HLA-Ⅰ类分子关系的研究[J]. 山东大学学报(医学版), 2006, 44(12): 1258-1260.

DENG Xiao-mei,WANG Jin-feng,ZOU Xiong,YU Xiu-ping,YANG Xiao-jing,ZHENG Gui-xi,LIU Min. Relationship between HBV infection and expression ofHLA molecules on host lymphocytes[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(12): 1258-1260.

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