关键词: 诱导分化 , 干细胞, 神经元样细胞, 胎血

Abstract: To study an experimental technology for repairing injured spine by transplantation of cord blood stem cells. Methods: (Human mesenchymal stem cells, hMSCs) were separated from cord blood by density gradient centrifuging and cultured in DMEM with 20% FBS. Amplified and taken the 5th generation to have an innoculation in the culture plate, they were put into the culture medium added inducer and cultivated at 37?℃, 5% CO2 in satured humidity. The neuron symbol NSE, NF and the astrocyte symbol GFAP were detected by immunohistochemical method, then under the microscope, 10 unwraped eyeshot was counted randomly and calculated the positive percentage of NSE, NF and GFAP denoting as ±s. Result: The hMSCs seemed like neurons and changed into taper cells with many long tuber and cytoplast shrinking to the nucleus which became denser and more refracting. Twentyfour hours after hMSCs were incubated in beforehand 3.63）％ of NSE, （33.46±3.21）％ of NF and （15.12±1.65）% of GFAP were found. Conclusion: Cord blood stem cells have a relatively strong ability of proliferation and a potent of differentiating into neuronlike cells at a certain condition.

Key words: Fetal blood, Stem cells, Neuronlike cells, Differentiation