JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Role of estrogen on the expression of KLK6 in the human ovarian cancer cell line HO8910

ZHANG Fang1，CHENG Ying-jian2，HU Cheng-jin2   

  1. 1.Department of Pathology, School of Medicine, Shandong University, Jinan 250012;2.Department of Clinical Laboratory, General Hospital of Jinan Military Command, Jinan 250031
  • Received:2006-09-25 Revised:1900-01-01 Online:2007-01-24 Published:2007-01-24
  • Contact: HU Cheng-jin

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Abstract: Objective： To investigate the effects of estrogen on the expression of KLK6 mRNA and protein in the human ovarian cancer cell line HO8910. Methods: HO8910 cells were incubated with 17-βE2 at different concentration (1×10-7、1×10-8、1×10-9 and 1×10-10mol/L) for 72 hours. Then the expression levels of KLK6 mRNA and protein were measured by real-time fluorensence quantitive RT-PCR and flow cytometry, respectively. Cell proliferation was measured by 3- (4, 5-dimethylthiazol-z-yl)-2, 5-dipheny tetrazolium blue (MTT) colorimetric assay, and the cell cycle was detected by flow cytometry. Results: Compared with the ethanol controls, the expression of KLK6 mRNA and protein in the HO8910 cell line accompanied by different concentrations of 17-βE2 increased (P<0.01 ) and the cell proliferation increased significantly， also the cell percentage of the G0/G1 phase decreased and that of the S phase and the G2/M phase increased. Conclusion: Estrogen up-regulates the expression of KLK6 mRNA and protein and stimulates HO8910 cell proliferation.

Key words: Ovarian neoplasms, Tissue kallikreins, Estrogens

CLC Number: 

  • R737.31
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