Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (6): 75-80.doi: 10.6040/j.issn.1671-7554.0.2019.351

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Simultaneous determination of five components in Cibotii Rhizoma by HPLC

SHI Yue, WANG Ning, LI Feifei, WANG Shuqi   

  1. School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To establish a validated HPLC method for quantitative analysis of kojic acid, 5-HMF, protocatechuic acid, maltol and protocatechuic aldehyde in Cibotii Rhizoma. Methods The samples were determined on a Shimatsu Shim-pak C18 column(4.6 mm×250 mm, 5 μm)by gradient elution with methanol as organic phase, 0.1% formic acid-ammonium formate aqueous solution as aqueous phase, at a flow rate of 1.0 mL/min. The detection wavelength was 280 nm for kojic acid, protocatechuic acid, maltol and protocatechuic aldehyde, while 260 nm for 5-HMF. The column temperature was controlled at 35 ℃. Results Kojic acid, 5-HMF, protocatechuic acid, maltol and protocatechuic aldehyde exhibited good linearity(r2=0.999 9, n=6)among the ranges of 0.53-21.17, 9.64-482.00, 2.52-100.88, 0.52-21.09, 0.52-20.87 μg/mL. The limit of quantitation(LOQ)/limit of detection(LOD)were 0.03/0.01, 0.20/0.05, 0.35/0.07, 0.05/0.02, 0.05/0.01 μg/mL, respectively. The average recoveries were 85.2%-102.8%. Conclusion C18 column was employed as stationary phase, dual wavelength detection and gradient elution HPLC method were used to determine the contents of five components in Cibotii Rhizoma. The method performs with high sensitivity and good accuracy, and can be used for quality control of Cibotii Rhizoma and its products.

Key words: Cibotii Rhizoma, HPLC, Content determination

CLC Number: 

  • R917
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