Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (6): 75-80.doi: 10.6040/j.issn.1671-7554.0.2019.351

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Simultaneous determination of five components in Cibotii Rhizoma by HPLC

SHI Yue, WANG Ning, LI Feifei, WANG Shuqi   

  1. School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To establish a validated HPLC method for quantitative analysis of kojic acid, 5-HMF, protocatechuic acid, maltol and protocatechuic aldehyde in Cibotii Rhizoma. Methods The samples were determined on a Shimatsu Shim-pak C18 column(4.6 mm×250 mm, 5 μm)by gradient elution with methanol as organic phase, 0.1% formic acid-ammonium formate aqueous solution as aqueous phase, at a flow rate of 1.0 mL/min. The detection wavelength was 280 nm for kojic acid, protocatechuic acid, maltol and protocatechuic aldehyde, while 260 nm for 5-HMF. The column temperature was controlled at 35 ℃. Results Kojic acid, 5-HMF, protocatechuic acid, maltol and protocatechuic aldehyde exhibited good linearity(r2=0.999 9, n=6)among the ranges of 0.53-21.17, 9.64-482.00, 2.52-100.88, 0.52-21.09, 0.52-20.87 μg/mL. The limit of quantitation(LOQ)/limit of detection(LOD)were 0.03/0.01, 0.20/0.05, 0.35/0.07, 0.05/0.02, 0.05/0.01 μg/mL, respectively. The average recoveries were 85.2%-102.8%. Conclusion C18 column was employed as stationary phase, dual wavelength detection and gradient elution HPLC method were used to determine the contents of five components in Cibotii Rhizoma. The method performs with high sensitivity and good accuracy, and can be used for quality control of Cibotii Rhizoma and its products.

Key words: Cibotii Rhizoma, HPLC, Content determination

CLC Number: 

  • R917
[1] 药典委员会. 中华人民共和国药典:一部[S]. 北京: 化学工业出版社, 2015: 224-225.
[2] 时圣明, 袁永兵, 兰新新, 等. 狗脊的化学成分及药理作用研究进展[J]. 药物评价研究, 2016, 39(3): 489-492. SHI Shengming, YUAN Yongbing, LAN Xinxin, et al. Research progress on chemical constituents in Cibotium barometz and their pharmacological activities[J]. Drug Evaluation Research, 2016, 39(3): 489-492.
[3] 白桐菲, 许枬, 贾天柱. 金毛狗脊炮制后化学成分研究[J]. 山西中医学院学报, 2008, 9(1): 41-42. BAI Tongfei, XU Nan, JIA Tianzhu. Study on the constituents of proceeded product of Cibotium baronetz[J]. Journal of Shanxi College of Traditional Chinese Medicine, 2008, 9(1): 41-42.
[4] Xie MP, Li L, Sun H, et al. Hepatoprotective hemiterpene glycosides from the rhizome of Cibotium barometz(L.)J. Sm[J]. Phytochemistry, 2017, 138: 128-133. doi:10.1016/j.phytochem.2017.02.023.
[5] 沙沂, 李文, 许枬, 等. 狗脊饮片多成分的1H-NMR分析[J]. 中国中药杂志, 2013, 38(3):371-375. SHA Yi, LI Wen, XU Nan, et al. Multi-component of Cibotium baronetz decoction pieces via 1H-NMR spectroscopic analysis[J]. China Journal of Chinese Materia Medica, 2013, 38(3): 371-375.
[6] 许枬, 步显坤, 周翎, 等. 烫狗脊中的酚性化合物研究[J]. 中国实验方剂学杂志, 2011, 17(8): 71-73. XU Nan, BU Xiankun, ZHOU Ling, et al. Chemical constituents from Cibotium baronetz[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2011, 17(8): 71-73.
[7] 许枬, 步显坤, 鞠成国, 等. 狗脊炮制品化学成分研究[J]. 中成药, 2009, 31(7): 1072-1074.
[8] 步显坤, 许枬, 袁野, 等.烫狗脊的质量标准研究[J]. 中成药, 2010, 32(7): 1266-1268
[9] Cuong NX, Minh CV, Kiem PV, et al. Inhibitors of osteoclast formation from rhizomes of Cibotium barometz[J]. J Nat Prod, 2009, 72(9): 1673-1677.
[10] Zhao X, Wu ZX, Zhang Y, et al. Anti-osteoporosis activity of Cibotium barometz extract on ovariectomy-induced bone loss in rats[J]. J Ethnopharmacol, 2011, 137(3):1083-1088.
[11] Mai W, Chen D, Li X. Antioxidant activity of rhizoma cibotii in vitro[J]. Adv Pharm Bull, 2012, 2(1): 107-114.
[12] 李军, 王振海, 王春田, 等. 狗脊及其炮制品对佐剂性关节炎大鼠血液流变学的影响[J]. 中国中药杂志, 2008, 33(17): 2170-2173. LI Jun, WANG Zhenhai, WANG Chuntian, et al. Influence of Cibotium barametz and its processed samples on haemorheology index in mice with adjuvant arthritis[J]. China Journal of Chinese Materia Medica, 2008, 33(17): 2170-2173.
[13] 鞠成国, 曹翠香, 史琳, 等. 狗脊及其炮制品和狗脊毛的镇痛、止血作用研究[J]. 中成药, 2005, 27(11): 1279-1281.
[14] 索天娇, 韩蕾, 贾天柱. 狗脊生、制品不同提取部位抗炎药效学实验研究[J]. 中华中医药学刊, 2012, 30(12): 2754-2756. SUO Tianjiao, HAN Lei, JIA Tianzhu. Pharmacodynamics study on the anti-inflammatory effects of different extraction parts from rhizoma cibotiis crude drugs and products[J]. Chinese Archives of Traditional Chinese Medicine, 2012, 30(12): 2754-2756.
[15] 许枬, 贾天柱. 烫狗脊炮制过程的化学反应及产物研究[J]. 中国中药杂志, 2011, 36(15): 2066-2070. XU Nan, JIA Tianzhu. Chemical reaction and products in processed Cibotium barometz[J]. China Journal of Chinese Materia Medica, 2011, 36(15): 2066-2070.
[16] 徐钢, 鞠成国, 于海涛, 等. 中药狗脊炮制研究进展[J]. 中国实验方剂学杂志, 2012, 18(5): 238-242. XU Gang, JU Chengguo, YU Haitao, et al. Advanced on Processing Cibotium barometz[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2012, 18(5): 238-242.
[17] 于海涛, 李慧, 章琦, 等. 狗脊炮制品中促进成骨细胞增殖分化成分的筛选[J]. 中成药, 2012, 34(6): 1139-1142. YU Haitao, LI Hui, ZHANG Qi, et al. Effective constituents screening of promoting osteoblastic differentiation from processed Cibotii Rhizoma[J]. Chinese Traditional Patent Medicine, 2012, 34(6): 1139-1142.
[18] 徐钢, 裴启洋, 鞠成国, 等. CCK-8法检测狗脊不同炮制品对成骨细胞的影响[J]. 中国中药杂志, 2013, 38(24): 4319-4323. XU Gang, PEI Qiyang, JU Chengguo, et al. Detection on effect of different processed Cibotium barometz on osteoblasts by CCK-8[J]. China Journal of Chinese Materia Medica, 2013, 38(24): 4319-4323.
[19] Masella R, Santangelo C, DArchivio M, et al. Protocatechuic acid and human disease prevention: biological activities and molecular mechanisms[J]. Curr Med Chem, 2012, 19(18): 2901-2917.
[20] 邢雅玲, 叶志华, 钟芝茵, 等. 原儿茶醛对脂多糖损伤的血管内皮细胞的作用机制研究[J]. 军事医学科学院院刊, 2008, 32(4): 344-347. XING Yaling, YE Zhihua, ZHONG Zhiyin, et al. Protection and related mechanisms of protocatechuic aldehyde against vascular endothelial cell injury induced by lipopolysaccharide[J]. Bulletin of the Academy of Military Medical Sciences, 2008, 32(4): 344-347.
[21] Zhou Z, Liu Y, Miao AD, et al. Protocatechuic aldehyde suppresses TNF-α-induced ICAM-1 and VCAM-1 expression in human umbilical vein endothelial cells[J]. European Journal of Pharmacology, 2005, 513(1/2): 1-8.
[22] 刘梅, 夏鑫华, 张志敏, 等. 丹参素、原儿茶醛、咖啡酸和丹酚酸B体外抗氧化活性比较研究[J]. 中药材, 2009, 32(2):265-267.
[23] Lee Y, Gao Q, Kim E, et al. Pretreatment with 5-hydroxymethyl-2-furaldehyde blocks scopolamine-induced learning deficit in contextual and spatial memory in male mice[J]. Pharmacol Biochem Behav, 2015, 134: 57-64. doi:10.1016/j.pbb.2015.04.007.
[24] Zhang JH, Di Y, Wu LY, et al. 5-HMF prevents against oxidative injury via APE/Ref-1[J]. Free Radic Res, 2015, 49(1): 86-94.
[25] Wang MY, Zhao FM, Peng HY, et al. Investigation on the morphological protective effect of 5-hydroxymethylfurfural extracted from wine-processed Fructus corni on human L02 hepatocytes[J]. J Ethnopharmacol, 2010, 130(2): 424-428.
[26] 耿放, 王喜军. 5-羟甲基-2-糠醛(5-HMF)在中药复方中的研究现状及相关药效探讨[J]. 世界科学技术-中医药现代化, 2005, 7(6): 52-56. GENG Fang, WANG Xijun. Present situation of study on 5-hydroxymethyl-2-furfural[J]. World Science and Technology-Modernization of Traditional Chinese Medicine, 2005, 7(6): 52-56.
[27] 黄洪新, 徐道华, 刘俊臣, 等. 地黄炮制前后5-羟甲基糠醛含量的研究[J]. 时珍国医国药, 2012, 23(4): 938-939. HUANG Hongxin, XU Daohua, LIU Junchen, et al. Content of 5 -HMF in rehmanniae radix after processing[J]. Lishizhen Medicine and Materia Medica Research, 2012, 23(4): 938-939.
[28] 曹岗, 季金玉, 丛晓东, 等. 玄参蒸制不同时间5-羟甲基糠醛的含量比较[J]. 中国新药杂志, 2012, 21(8): 936-937, 942. CAO Gang, JI Jinyu, CONG Xiaodong, et al. Changes of 5-hydroxymethyl furfuraldehyde contents in Radix Scrophulariae steamed for different times[J]. Chinese Journal of New Drugs, 2012, 21(8): 936-937,942.
[29] 宋志前, 曾林燕, 王淳, 等. 鲜地黄加工炮制后新成分含量变化研究[J]. 世界科学技术-中医药现代化, 2014, 16(2): 346-351. SONG Zhiqian, ZENG Linyan, WANG Chun, et al. Content changes of chemical constituents produced in fresh radix rehmanniae after processing[J]. World Science and Technology-Modernization of Traditional Chinese Medicine, 2014, 16(2): 346-351.
[30] 方应权. 巫溪大宁党参不同的炮制方法对5-HMF的含量影响[J]. 中国中医药现代远程教育, 2014, 12(6): 151-152. FANG Yingquan. Effect on the content of 5-HMF by the different processing in the Codonopsis pilosula[J]. Chinese Medicine Modern Distance Education of China, 2014, 12(6): 151-152.
[31] 徐男, 时海燕, 孙蓉. 5-羟甲基糠醛的含量与大黄炭炮制程度及质量相关性研究[J]. 时珍国医国药, 2015, 26(9): 2152-2154.
[32] 徐钢, 孙娜, 贾天柱, 等. 狗脊不同炮制品水煎液抗维甲酸致雄性大鼠骨质疏松症研究[J].中国中药杂志, 2014, 39(6): 1011-1015. XU Gang, SUN Na, JIA Tianzhu, et al. Study on decoctions effect of different processed rhizomes of Cibotium barometz on retinoic acid induced male rats osteoporosis[J]. China Journal of Chinese Materia Medica, 2014, 39(6): 1011-1015.
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