人参皂苷对金黄色葡萄球菌生物被膜形成的影响

doi:10.6040/j.issn.1671-7554.0.2018.013

Abstract

Abstract: Objective To investigate the effect of ginsenoside Rh2 on the biofim formation of Staphylococcus aureus(S.aureus). Methods The biofilm was constructed by using 96-well cell culture plate to study the effect of Rh2 on the biofilm formation of S.aureus, and the effect of Rh2 on the expression of adhesin-related genes of S.aureus was evaluated by RNA extraction and real-time quantitative PCR. The combination of 96-well cell culture plate with crystal violet staining was used to explore the synergistic antibiofilm effects of Rh2 in combination with ciprofloxacin(CIP), gentamicin(GEN)and vancomycin(VAN). Results Rh2 had no effect on the cell growth of S.aureus, while could significantly reduce the biofilm formation(P<0.05)at the concentration of 5 μg/mL. Rh2(10 μg/mL)could significantly inhibit the gene expressions of icaA, icaC and icaD(P<0.05), and promote the inhibitory effects of CIP, GEN and VAN on S.aureus biofilm formation(P<0.05). Conclusion Ginsenoside Rh2 can inhibit the biofilm formation of S. aureus by inhibiting the expressions of adhesion-related genes icaA, icaC and icaD. Rh2 can also promote the inhibitory effects of CIP, GEN and VAN on the biofilm formation of S.aureus.

Key words: Staphylococcus aureus, Ginsenosides, Rh2, Biofilm, Synergistic effect

CLC Number:

R378.1+1

AN Jihong, L(¨overU)Weiling, ZHANG Yongzhou, HAN Shanshan, LIU Qiang. Effects of ginsenosides on the biofilm formation of Staphylococcus aureus [J].Journal of Shandong University (Health Sciences), 2018, 56(7): 28-32.

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Effects of ginsenosides on the biofilm formation of Staphylococcus aureus

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