NR2B通过mBDNF参与肠易激综合征内脏高敏感的发生

doi:10.6040/j.issn.1671-7554.0.2016.1674

Abstract

Abstract: Objective In the nervous system, N-methyl-D-aspartate receptors(NMDARs)subunit NMDA receptor 2B(NR2B)is involved in visceral hypersensitivity. This study aims to investigate if colonic mucosal NR2B contributes to visceral hypersensitivity in irritable bowel syndrome via mature brain-derived neurotrophic factor(mBDNF). Methods A total of 32 participants were included. Colonic mucosal NR2B subunits and mBDNF expressions were detected. The relationship between the NR2B and mBDNF expressions was analyzed with Pearson correlation analysis. The relationship between mBDNF expression and abdominal pain scores was analyzed with Spearman correlation analysis. After administration of NMDARs agonist, NMDARs antagonist and NR2B subunit antagonist, the expression of mBDNF in cell culture supernatant was assessed with ELISA. Results NR2B and mBDNF expressions were significantly increased in colonic mucosa of IBS-D patients(NR2B: 90.22±30.79 vs 63.47±28.19, P=0.016; mBDNF: 89.91±20.95 vs 56.53±22.25, P<0.001). NR2B expression was positively correlated with mBDNF expression. Furthermore, mBDNF expression was positively correlated with abdominal pain scores. The activation of NMDAR induced mBDNF expression, which could be completely inhibited by NMDARs antagonist and NR2B subunit antagonist. Conclusion NR2B contributes to visceral hypersensitivity in IBS-D via mBDNF.

Key words: Irritable bowel syndrome, NR2B, Mature brain-derived neurotrophic factor, Visceral hypersensitivity

CLC Number:

R574

ZHANG Wenxue, XU Lidong, ZHANG Mingming, GUO Chuanguo, ZUO Xiuli. NR2B contributes to visceral hypersensitivity in irritable bowel syndrome via mBDNF[J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(7): 17-22.

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NR2B contributes to visceral hypersensitivity in irritable bowel syndrome via mBDNF

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