JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (12): 20-23.doi: 10.6040/j.issn.1671-7554.0.2014.172

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Hypoglycemic mechanism of volatile oil of plateau plant Brassica rapa L. in hyperlipidemic and hyperglycemic mice

CHEN Xianghong, LIU Yan, WENG Yuxin, KANG Wenjuan, YANG Shibing   

  1. Department of Pharmacy, Medical College of Qinghai University, Xining 810001, Qinghai, China
  • Received:2014-03-27 Revised:2014-10-29 Published:2014-12-10

Abstract: Objective To explore the hypoglycemic mechanism of plateau plant Brassica rapa L. (turnip) volatile oil in an animal model of diabetic mice. Methods The volatile oil was extracted using supercritical carbon dioxide extraction method. The diabetic mice model was built by feeding high-fat and high-carbohydrate diet for 4 weeks then intraperitoneal injection of streptozotocin (STZ) 100 mg/kg for 3 days. The mice with fasting blood glucose in tail vein being greater than 11.0 mmol/L were defined as successful experimental models. Fifty mice were randomly divided into control group, model group, high dose of turnip volatile oil group, low dose of turnip volatile oil group, and positive control (metformin hydrochloride) group, 10 in each group. The mice were perfused for 14 days at one time per day. The blood was collected from tail vein and the blood glucose level was measured before and after building the models, and at day 3, 7, 14 after medication. The blood samples were taken from the mice's eyeballs and the glycosylated hemoglobin (HbA1c), triglyceride (TG), total cholesterol (TC), and serum insulin (INS) levels were measured. The fresh spleen and thymus gland were cut and weighed to compute organ index. Results Both high dose and low dose of turnip volatile oil had significant influences on the type 2 diabetic model mice, including reduced blood glucose (P<0.01) and HbA1c levels (P<0.05), increased INS level (P<0.05), and decreased TG and TC levels (P<0.01). The turnip volatile oil could protect the thymus in diabetic mice, and enhance the immune ability. Conclusion Turnip volatile oil can reduce hyperglycemia significantly in diabetic model induced by STZ, which may due to the intervention of the biosynthesis of HbAlc and increase of INS level.

Key words: Serum insulin, Tri-glyceride, Glycosylated hemoglobin, Total cholesterol, Brassica rapa L. (turnip) volatile oil

CLC Number: 

  • R285.5
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