JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (7): 61-64.doi: 10.6040/j.issn.1671-7554.0.2014.1017

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Effect of Gonadotrophin on the in vitro maturation of immature oocytes from controlled ovarian stimulation

SONG Xinggui, CHEN Zijiang, MA Shuiying, WU Keliang, LI Mei   

  1. Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University; National Research Center for Assisted Reproductive Technology and Reproductive Genetics, China; Key Laboratory for Reproductive Endocrinology of Ministry of Education; Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan 250021, Shandong, China
  • Received:2014-12-31 Revised:2015-03-26 Published:2015-07-10

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Abstract: Objective To evaluate the effect of Follicle-stimulating hormone (FSH) and Human chrorionic gonadotrophin (HCG) on in vitro maturation of immature oocytes obtained from controlled ovarian stimulation (COH) cycles. Methods MⅠ and GV stage oocytes were collected from COH cycles for intracytoplasmic sperm injection (ICSI) treatment. All of these immature oocytes were divided into two groups (donadotrophin treatment group and non-donadotrophin treatment group) and cultured for 24 to 28 hours in insemination media. The donadotrophin group was treated with 0.075 IU/mL FSH and 0.15 IU/mL HCG. Matured oocytes were given ICSI. The rates of maturation, normal fertilization, cleavage, and high-quality embryo were recorded and compared. Results For MⅠ oocytes, there were no difference between the two groups in maturation rate, fertilization rate, cleavage rate and high-quality embryo rate (P>0.05). For GV oocytes, high-quality embryo rate in donadotrophin treatment group was higher than that in non-donadotrophin treatment group (31.3% vs 4.2%, P<0.05). Conclusion Immature oocytes from COH can mature in vitro whether hormones are added or not. During the maturation of the GV stage oocytes, donadotrophin can increasethe developmental potential of oocytes.

Key words: In vitro maturation, Intracytoplasmic sperm injection, Immature oocyte, Follicle-stimulating hormone, Human chrorionic gonadotropin

CLC Number: 

  • R711.6
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