Abstract:

Objective  To study the expression of microRNA-150(miR-150) in colon cancer cell lines and colorectal cancer (CRC) tissues, and evaluate its clinicopathological significance. Methods  miR-150 was examined in eight colon cancer cell lines, 45 CRC tissues and their matched normal tissues by real-time quantitative reverse transcription PCR (qRT-PCR). The relationships between miR-150 and clinicopathological information were analyzed. Results  Expression of miR-150 in eight colon cancer cell lines was lower than normal tissues. Moreover, miR-150 expression in tumor tissues was much lower than their matched normal tissues (P<0.05). Low miR-150 level was strongly associated with the differentiated degree (P<0.05) and pathological type (P<0.01). Conclusion  miR-150 is down-regulated not only in colon cancer cell lines but in tumor tissues, and its low expression is associated with the differentiation and pathological type. miR-150 might play a role in the course of CRC diagnosis and treatment.

Key words: microRNA-150; Colorectal cancer; Real-time quantitative reverse transcription PCR; Mucinous cancer