Abstract:

Objective  To confirm that diabetes has an influence on in-situ saphenous vein, and to explore the effects of TNFAIP8 and TIPE2 on in-situ saphenous vein of diabetes mellitus. Methods  The residual fresh great saphenous veins specimen were gotten from the patients during CABG surgery, which were divided into experimental group (with type 2 diabetes mellitus, DG) and control group (without type 2 diabetes mellitus, NG). Each specimen was 5 cm long for further experiments as follows: vascular physical properties, biological stability, microscopic structure and expression, distribution of inflammatory proteins. Results  Physical properties of vessels: water content: NG＞DG(P＞0.05); vascular heat shrink temperature: NG＞DG(P＜0.05); vascular rupture strength:NG＞DG(P＜0.01); vascular biology stability index:  NG＞DG(P＜0.01); elastic fiber content(RE):NG＞ DG(P＜0.05);  collagenous fiber content(RC): DG＞NG(P＜0.05); tube-wall stiffness (C/E):  DG＞NG(P＜0.01); connective tissue stroma′s volume density in circular smooth muscle (CC/MC): DG＞NG(P＜0.01); TNFAIP8 and TIPE2: DG＞NG. Conclusion  The diabetes mellitus has an influence on in-situ saphenous vein, while the TNFAIP8 and TIPE2 have an influence on in-situ saphenous vein of diabetes mellitus.

Key words: Coronary artery disease; Coronary artery bypass grafting; Diabetes mellitus; In-situ saphenous vein; Vein-grafts