Abstract:

Objective     To investigate the effect of basil polysaccharide on the invasiveness of MDA-MB-231 cells. Methods    MDA-MB-231 cells cultured in vitro were divided into the experimental group and control group. The control group was not treated, while the experimental group was treated with 100μg/mL or 300μg/mL basil polysaccharide. The invasiveness of the cells was detected with transwell assay. The expressions of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and membrane-type 1 matrix metalloproteinase (MT1-MMP) were tested with RT-PCR and Western blotting. Results   Basil polysaccharide suppressed the invasion of MDA-MB-231 cells (P<0.01), decreased the mRNA expression of MT1-MMP (P<0.05), but had no effect on the mRNA expression of MMP-2 and MMP-9. Conclusion    Basil polysaccharide inhibits the invasiveness of MDA-MB-231 cells by reducing the expression of MT1-MMP.

Key words: Basil polysaccharide; Tumor invasion; Membrane-type 1 matrix metalloproteinase; Breast neoplasm