Abstract:

Objective     To observe expression of autophagy-related gene Beclin1on second-degree scald tissues in rats, and to investigate the relationship of autophagy and scald tissues. Methods    Sixteen  Wistar rats were randomly divided into 2 groups:the normal group (the control group) and the scald group (the experimental group), 8 rats in each group. Second-degree scald models were established in experimental group, and scald tissues were obtained on day 1, 3 and 7 after scald. The rat skin in each group was evaluated by histologically examination. Expression of Beclin1 in each group was detected by the immunohistochemiscal technique and conducted by image processing techniques. Statistical analyses were performed. Results   Expression of Beclin1 was positive in the experimental group, but negative in the control group(P＜0.05). Conclusion    Expression of Beclin1 in the experimental group was significantly higher than that in the control group, and autophagy was speculated to participate repair process of second-degree scald.

Key words: Autophagy; Gens, Beclin1; Rats,  Wistar; Scald