Abstract:

Objective   To study the effects of inflammation on the growth of tumor in fibrosarcomabearing mice and the possible mechanism. Methods   C57BL/6 mice were randomly divided into 5 groups： the normal control group (PBS group), the lipopolysaccharide group (LPS group), the fibrosarcoma group (PC group), the LPS + fibrosarcoma group (LC group), and the LPS + fibrosarcoma + celecoxib group (Cele group). PBS buffer was intraperitoneally injected to the PBS group mice, and the remaining groups were given lipopolysaccharide (LPS) to establish the inflammation model. The mice of the LPS and PBS groups were killed on the fifth day， and the rest were subcutaneously inoculated with fibrosarcoma cells. Meanwhile the Cele group mice were given celecoxib and the mice were sacrificed 21 days after the inoculation. The general conditions and tumor volumes of the mice were compared. Mice were sacrificed, and the profile changes and the pathological characteristics of the lung tissues were studied. Tumor microvascular density and vessel phenotype of PC group and LC group mice were compared by CD31 staining. Results   Compared with the PBS group, the lung vascular permeability and the tissue fluid exudation increased, and a large number of red blood cells and inflammatory cells were observed in the LPS group. Compared with the PC group， the tumors in the LC group grew faster, the microvascular density increased and the blood vessels were in disorder. Conclusion   The inflammatory response promotes the growth of fibrosarcoma possibly by the promotion of the tumor angiogenesis. Antiinflammatory treatment can inhibit the growth of fibrosarcoma.

Key words: Fibrosarcoma; Inflammation; Angiogenesis; Lipopolysaccharide; Mice