Abstract:

Objective   To investigate the renoprotective effects of Dendrobium nobile Lindl (DNL) on nonenzymatic glycation and oxidative stress in the diabetic rat kidney. Methods   The diabetes mellitus (DM) rat model was induced by streptozotocin. All rats were divided into six groups: the normal control group(N group), diabetic control group(DM group), DNL low dose group(DNLL group), DNL moderate dose group(DNLM group), DNL high dose group(DNLH group) and AG control group(AG group), and administrated for 12 weeks. The serum glucose levels, 24h urinary albumin, serum urea, creatinine, advanced glycation end products (AGEs), urine creatinine, renal total superoxide dismutase (SOD) and malondialdehyde (MDA) were measured. The ultrastructural morphology was observed by the electron microscope. Results   The serum glucose levels, serum urea, 24h urinary albumin, serum and renal AGEs, and renal MDA significantly increased in the DM group compared with N group(P＜0.05). The levels of creatinine clearance rate (Ccr) and total SOD significantly reduced in the DM group compared with N group(P＜0.05). The mesangium expansion and basement membrane thickness significantly increased in the DM group. DNL significantly decreased the serum glucose levels, serum urea, 24h urinary albumin, serum and renal AGEs and renal MDA(P＜0.05), and significantly increased the levels of Ccr and total SOD(P＜0.05). The mesangium expansion and basement membrane thickness were significantly improved by DNL. Conclusion   DNL can decrease serum glucose, inhibit AGEs formation, attenuate oxidative stress, and retard the occurrence and development of diabetic nephropathy.

Key words: Dendrobium nobile Lindl; Diabetes mellitus; Advanced glycation end products; Oxidative stress