Abstract:

Objective   To establish a high performance liquid chromatography with fluorescence detector for the determination of the benzo(a)pyrene(B(a)P) level in the cooking oil. Methods   The difference of B(a)P content in edible oil in Jinan market was investigated by the SHIMADZU shim-pack VP-ODS (250mm×4.6mm, 5μm) column. The mobile phase was methanol-water(90∶10, V/V ), and the flow rate was 1. 0mL/min. The samples were treated by the non-bonded silica solid phase extraction(SPE) column, and then detected at the 285nm excitation wavelength and 406nm emission wavelength. Results   The lowest limit of determination was 0.039μg/kg (S/N=3). The recovery rate was 85.8%-90.7%,  and the intra-day and inter-day relative standard deviations were 2.9%-4.1% and 3.7%-5.3%. Conclusion   This method is high sensitive, good selective and quantitative accurate, which is suitable for the B(a)P determination in the cooking oil.

Key words: High-performance liquid chromatography； Fluorescence detector； Cooking oil； Benzo(a)pyrene； Solid phase extraction