Abstract:

Objective   To explore the protective effect of resveratrol on Aβ25-35-induced injury in PC12 cells. Methods   PC12 cells were treated with Aβ25-35 of different concentrations to establish the Alzheimer’s disease models. Resveratrol of different concentrations were added into culture medium to detect the protection. The changes of cell morphology and neurite outgrowth were observed under a microscope. Cell activity was identified by MTT analysis. Lactate dehydrogenase (LDH) activity was identified by ELIASA. Cell apoptosis was detected by Giemsa staining and annexinV-FITC/PI double staining flow cytometry. Results   PC12 cells treated with Aβ25-35 suffered axonal degeneration in the distal end of the axons at 24h. The axons were disrupted at 48h. The protection group with the pre-incubation of resveratrol significantly delayed axonal injury. The axons remained relatively good structures at 24h, and the axons only suffered minor fractures at 48h. The axon length and D(λ)value of the protection group were significantly larger than those of the injury group(P<0.01). The apoptosis and LDH activity of the protection group were significantly lower than those of the injury group (P<0.01). Conclusion   Resveratrol plays an important role in protecting Aβ25-35-induced injury in PC12 cells.

Key words: Apoptosis; Resveratrol; Amyloid beta-protein; PC12 cells