Abstract:

Objectives   To evaluate the advantages and disadvantages of different methods of establishing experimental dog models with acute pulmonary hypertension, and to evaluate the hemodynamic changes of pulmonary hypertension caused by the suture line method. Methods  Fifteen anesthetized mongrel dogs were randomly divided into the glucan group, suture group and thrombosis group, five in each group. The injection of glucan, suture line and autologous blood clots through the pulmonary artery catheter established corresponding animal models of acute pulmonary embolism. The cardiac catheter indexes RVSP were measured to evaluate the advantages and disadvantages of the different experimental methods. The measured cardiac catheterization indexes and the capacity index measured by echocardiography were used to study hemodynamic changes in the suture line group. Results   The glucan group and suture group showed more repeatability (stability) than that of thrombosis group (P<0.05) and the suture group showed no more persistence than that of the glucan group (P>0.05). Moderate increasing of pulmonary artery pressure and right ventricle pressure load resulted in expansion of pulmonary artery and right ventricle, mild decrease of aortic systolic pressure and cardiac output(P>0.05). Severe pulmonary hypertension and right ventricle pressure load increase resulted in further expansion of the pulmonary artery and right ventricle, severe decrease of aortic systolic pressure and cardiac output(P<0.05). Conclusion   The method using the suture line is simpler and more  effective to establish the acute pulmonary hypertension model and research hemodynamic changes. Biventricular hemodynamic changes derived from acute pulmonary hypertension will be helpful for clinical evaluation and treatment of acute pulmonary embolism.

Key words: Acute pulmonary embolism; Pulmonary hypertension;  Models, animal； Hemodynamics