Abstract:

Objective   To explore the effect of feitongkoufuye treating pulmonary interstitial fibrosis on neutrophil elastase(NE). Methods   40 SD rats were randomly divided into the model group, the treatment group, the hormone group and the normal control group, 10 rats in each group. BLEMA5 was used to establish a rat model of pulmonary interstitial fibrosis. The treatment group was given Feitongkoufuye (250mg/kg) by gavage; the hormone group was given hydrocortisone (25mg/kg) by intramuscular injection; the control group was given 0.9% saline solution (2mL/kg) by disposable tracheal instillation. Pathological changes were observed by HE and VG staining, serum cell counting and classification were performed, and NE contents were determined by ELISA method. Results   Compared with the normal control group,  alveolitis and pulmonary fibrosis in the model group were more severe, and the deposition of collagen, serum total cell number, cytological classification proportion, and the content of serum NE were significantly increased (P<0.01). Compared with the model group and treatment group, alveolitis and fibrosis in the treatment group were slighter, and the deposition of collagen, total number of cells, cytological classification proportion, and content of serum NE were significantly reduced (P<0.01). Conclusion   Feitongkoufuye significantly reduces inflammatory cell exudation and NE content, thereby alleviating rat alveolitis and pulmonary interstitial fibrosis.

Key words:  Pulmonary fibrosis; Feitongkoufuye; Cell count; Leukocyte elastase