Abstract:

Objective    To detect ATP1A2 gene mutation in an alternating hemiplegia of childhood(AHC) family，in order to find a new genetic mutation leading to AHC. Methods   DNA was extracted from 5 patients from a AHC family，then the primer was designed according to the mutation domain for PCR, and PCR DNA products were sequenced for the mutation. Results   Compared with the normal ATP1A2 gene sequence, no mutation was found in the detected gene sequence. There was no obvious doublet at 1,237c and other sites in the gene sequencing figure. There was no gene mutation in DNA samples from the 5 patients. Conclusion   The relationship between ATP1A2 gene mutation and AHC should be confirmed by further research.

Key words: Hemiplegia; Child; Genes, ATP1A2; Classic migraine