JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (10): 77-.

• Articles • Previous Articles     Next Articles

A proteomics study of docetaxel-induced apoptosis in
prostate cancer PC3 cells

XIAO Pan1, ZHANG Huai-qiang1, CUI Ya-zhou2, ZHAO Sheng-tian1, MA Tian-jia1, ZHOU Chun-wen1   

  1. 1. Department of Urology, The Second Hospital of Shandong University, Jinan 250033, China;
    2. Shandong Medicinal Biotechnology Center, Shandong Academy of Medical Sciences, Jinan 250062, China
  • Received:2012-05-30 Online:2012-10-10 Published:2012-10-10

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Abstract:

Objective   To investigate the preliminary molecule mechanism of docetaxel resistance in prostate cancer cells by comparing the differential protein expressions before and after docetaxel-induced apoptosis in androgen independent prostate cancer PC3 cells. Methods   The sulforhodamine B method was used to detect the inhibition effect of different concentrations of docetaxel on the proliferation of PC3 cells. Differential gel electrophoresis (DIGE) was adopted to separate and identify the differential proteins before and after docetaxel-induced apoptosis. Results   The median inhibitory concentration IC50 was 20nmol/L. Eighteen significant differential protein spots were defined by DIGE. Eight proteins were up-regulated while ten proteins were down-regulated. Conclusion   Eighteen significant differential protein spots were identified by using the proteomics method. It was believded that various differential proteins might be involved in the docetaxel resistance in androgen independent prostate cancer therapy.

Key words: Prostate neoplasms; Docataxel; Proteomics; Differential gel electrophoresis; Apoptosis

CLC Number: 

  • R737.25
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