Abstract:

Objective   To explore the expressions of p38MAPK, NF-κB and ICAM-1 in the hippocampus of the rabbits suffering from the cerebral vasospasm (CVS) caused by subarachnoid hemorrhage (SAH). Methods   The 60 New Zealand purebred healthy rabbits were divided into control group (n=5),NS injection group (n=5), SAH group (n=30) and the intervention group (n=20), then the SAH group was divided into 6 subgroups (1、3、5、7、9、11d）according to the days after having got the SAH, the intervention group was divided into 4 subgroups which were treated by the NS , the ICAM-1 monoclonal antibody, the NF-κ B antagonist and the p38MAPK inhibitor respectively. The CVS models caused by the SAH were made by the secondary blood injection into the major cistern. Then the hippocampus was separated and the Immuno-histochemistry was employed to observe the change of the expression of p38MAPK, NF-κB and ICAM-1 in it. Results   The slight dyeing of p38MAPK, NF-κB and ICAM-1 was observed by immuno-histochemistry in hippocampus of the rabbits in the control group and NS injection group. With the CVS aggra vating, theexpression of the ICAM-1 was enhanced gradually and got the top in the 7th day. The expression of the p38MAPK、NF-κB also had the trend, but got the top in the 5th day, then decreased gradually. After the treatment of the NF-κ B antagonist, the dyeing signal of the p38MAPK、NF-κB was weakened obviously, and the ICAM -1  was only expressed slightly, then after the treatment of the ICAM1 monoclonal antibody, the p38MAPK、NF-κB were expressed strongly while the ICAM -1 was expressed slightly. Conclusion   There was the immuno-inflammatory reaction related to the above factors in the hippocampus of the rabbits having got the CVS caused by SAH. The immuno-inflammatory reaction after the craninocerebral trauma could be controlled better if the expression of the above factors were inhibited.

Key words: Hippocampus; p38 Mitogen-activated kinase protein; Nuclear transcription factorκB; Intercellular adhesion molecule-1; Subarachnoid hemorrhage; Cerebral vasospasm