JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2011, Vol. 49 ›› Issue (6): 110-114.
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KONG Hai-li, MA Dao-xin, ZHANG Jingjing, WANG Hui-jun, LIU Chuan-fang
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Objective To investigate expressions and relationship of PDCD4 and TGF-β1 in the bone marrow of patients with acute myeloid leukemia(AML). Methods RT-PCR, ELISA and Western blotting were used to detect mRNA and protein expression of PDCD4 and TGF-β1 in 30 newly diagnosed AML patients, 30 AML patients who had reached complete remission (AML-CR) and 20 patients with iron deficiency anemia(IDA)as controls. The correlation between PDCD4 and TGF-β1 was further analyzed. Results The results of RT-PCR indicated that mRNA expression of PDCD4 and TGF-β1 in the newly diagnosed AML group was significantly lower than the control group(P<0.05). Though the AML-CR group was higher than the AML group, the difference was not significant(P>0.05).The results of ELISA showed that the level of TGFβ1 in the newly diagnosed AML group was significantly lower than the other two groups(P<0.05). The results of Western blotting indicated that the expression of PDCD4 protein in the AML group was significantly lower than the control group and the AML-CR group (P<0.05). PDCD4 and TGF-β1 expression showed a positive correlation(P<0.05). Conclusion PDCD4 and TGF-β may play important roles in the occurrence and development of AML, and might have a synergistic effect.
Key words: Acute myeloid leukemia; Programmed cell death 4; Transforming growth factor β1
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KONG Hai-li, MA Dao-xin, ZHANG Jingjing, WANG Hui-jun, LIU Chuan-fang. Expression and significance of PDCD4 and TGF-β1 in patients with acute myeloid leukemia[J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(6): 110-114.
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