Abstract:

Objective     To explore the role and mechanism of  the Wnt/β-catenin pathway on proliferation of embryonic carcinoma cells(EC)． Methods     P19 cells， a mouse EC cell line， were divided into four groups： the normal control group(con)， the RA group (medium with RA)， the SB group(medium with GSK3β inhibitor SB216763)， and SB+RA group (medium with both SB216763 and RA)． At different culture times， immunofluorescence staining， RT-PCR and Western blotting were employed to observe expression of β-catenin， Oct4 and C-myc. BrdU labeling and MTT were used to measure cell proliferation. Results      The addition of SB216763 improved the nuclear location of β-catenin， high expression of Oct4 and C-myc， blocked P19 cell differentiation induced by RA， and dramatically promoted P19 cell proliferation． Conclusion      Activation of the Wnt/β-catenin pathway could promote proliferation and inhibit differentiation of mouse EC cells by upregulating C-myc expression．

Key words: Embryonic carcinoma cells； β-catenin； C-myc； Proliferation