JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2011, Vol. 49 ›› Issue (4): 85-89.

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Relationship of expressions of PDGFR-β and podoplanin with cancer-associated fibroblasts and  lymphangiogenesis in NSCLC

LIN Wen-li1, LIU Jie2, LI Zhe3, ZHANG Nan2, LIU Chuan-yong2, LIU Jin-feng1, SUN Yu-ping2   

  1. 1. School of Medicine, Shandong University, Jinan 250012, China；
    2. Department of Oncology， Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China；
    3. Department of Pathology， Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China
  • Received:2011-01-12 Online:2011-04-10 Published:2011-04-10

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Abstract:

Objective    To evaluate the relationship of expressions of plateletderived growth factor receptor(PDGFRβ) and podoplanin with cancerassociated fibroblasts(CAFs) and lymphangiogenesis in nonsmall lung cancer（NSCLC）. Methods     Applying the immunohistochemical Elivision method, CAFs were labeled by αsmooth muscle actin(αSMA), and lymphatic vessels and expressions of PDGFR-β and podoplanin were detected in 80 cases of NSCLC and 20 adjacent normal tissues. The lymphatic microvessel density (LMVD) was counted by D2-40 as the specific marker of lymphatic endothelium. Relationships of expressions of PDGFR-β and podoplanin with CAFs, LMVD and clinicopathological features of NSCLC were then analyzed. Results    Expressions of PDGFR-β and podoplanin in the interstitium of NSCLC were both higher than those in adjacent normal tissues ［PDGFR-β:  46.35%(39/80) vs 20%(4/20)；podoplanin： 55.00%(44/80) vs 10%(2/20), both P＜0.05］. Expressions of PDGF-β and podoplanin were closely related to CAFs. Expression of PDGFR-β in the interstitium of NSCLC was significantly correlated with that of podoplanin（r=0.380, P＜0.05）. LMVD in NSCLC was significantly higher than that in adjacent normal tissues (U=93.00, P＜0.05). LMVD was significantly higher in NSCLC with lymph node metastasis and PDGFR-β-or podoplanin-positive expression than that  with no lymph node metastasis and negative expression［lymph node metastasis：（26.06+16.41） vs (17.26+12.97), t=2.582，P＜0.05；PDGFR-β:（27.63+15.46） vs (17.47+14.68), t=3.01，P＜0.05； podoplanin：（27.70+17.68） vs (15.96+9.84), t=3.74，P＜0.05］. Expression of PDGFR-β in the interstitium of NSCLC was correlated with differentiation (P＜0.05). Expression of podoplanin in the interstitium of NSCLC was correlated with differentiation and lymph node metastasis（both P＜0.05）. ConclusionIn     NSCLC, expressions of PDGFR-β and podoplanin are closely related to CAFs, and can promote lymphangiogenesis.

Key words: Cancer-associated fibroblasts; Platelet-derived growth factor receptor-β; Podoplanin; Lymphangiogenesis

CLC Number: 

  • R734.2
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