Abstract:

Objective    To investigate the effect of interlukin-17(IL-17) on proliferation and the cell cycle of hepatic stellate cells（HSC）. Methods    HSC were treated with IL-17(200, 400, 600, 800, or 1000μg/L)for 24, 48 and 72 hours. HSC in the blank control group were treated without IL-17. The effects of IL-17 on proliferation and the cell cycle were detected by MTT assay and flow cytometry, respectively. Results    The quantity of the cells was associated with the dose of IL-17 and the incubation time. IL-17(200μg/L) had no significant stimulation effect on HSC(P>0.05), while IL-17(800μg/L) for 72h had the most significant stimulation effect (P<0.01). IL-17(1000μg/L) could inhibit the proliferation of HSC(P<0.01). IL-17 could change the distribution of cells at different phases of the cell cycle, promoting the G1 phase cells entering into the S phase. IL-17(1000μg/L) could block cells at the G1 phase(P<0.01).  Conclusions    IL-17 could stimulate the proliferation of HSC, which is associated with the dose of IL-17 and the incubation time, and it could promote G0/G1 phase cells entering into the S phase.

Key words: Interlukin-17； Cell proliferation； Cell cycle； Hepatic stellate cells, Laparoscopic unilateral adrenalectomy; Nodular adrenal hyperplasia; Hypertension; Primary aldosteronism