Abstract:

Objective   To investigate Th17 existence and infiltration in glioma by testing Th17 correlation factors expression. Methods   The presence of CD4+ and IL-17 were measured by immunohistochemistry in 34 human glioma tissues and 10 normal brain tissues. Expression levels of both IL-17mRNA and RORγtmRNA were detected by QRT-PCR technology. Results   Neither CD4+ nor IL-17 had positive staining in the 10 normal brain tissues. In the low grade group (WHOⅠ-Ⅱ), there were low expression levels of both CD4+ and IL-17. Compared with those of the low-grade group, there were obviously higher expression levels of both CD4+ and IL-17 in the highgrade group (WHOⅢ-Ⅳ). There was significantly statistical difference among the comparisons(P<0.05，P<0.05，P<0.01). The QRT-PCR result showed that expression levels of IL-17AmRNA and RORγtmRNA had a positive correlation with the malignancy of human glioma. There was significant statistical difference among the comparisons of the three groups(P<0.05，P<0.05，P<0.01). Conclusion   There is Th17 infiltration in glioma,whose expression level has a positive correlation with the malignancy of human glioma.

Key words: Glioma; Th17cells; Interleukin-17; Retinoic acidrelated orphan receptor