Abstract:

Objective    To investigate methylation of the P15INK4B gene in lymphoma cell line Raji cells and to evaluate the effects of decitabine on demethylation of P15INK4B in human lymphoma cell line Raji cells and on proliferation of Raji cells. Methods    Raji cells were treated with decitabine, and the effect of decitabine on proliferation of Raji cells wth trypan blue exclusion was investigated and the apoptosis of cells stained by Annexin /Ⅴ-FITC was determined. The mRNA expression of P15INK4B in cells treated with decitabine was determined by reverse transcription-polyerase chain reaction (RTPCR). Methylation of the P15INK4B gene in Raji cells was determined by PCR using methylation specific primer (MSP). Results    Decitabine inhibited the Raji cell growth and promoted apoptosis of Raji cells, and up-regulated the  P15INK4B mRNA expression by demethylation of the p15INK4B gene. Conclusion  P15INK4B gene of Raji cells was methylated and down-regulated. Decitabine inhibited Raji cell growth by demethylation of P15INK4B.

Key words: Lymphoma； P15INK4B； Decitabine; Methylation