Abstract:

Objective   To establish Alzheimer disease（AD） models of Wistar rats using β-amyloid1-42 (Aβ1-42) and study the effect of diazoxide on the change of apoptotic factors and the ethology of rat AD models. Methods  Aβ1-42 was injected into the bilateral ventricles of all the rats, and two weeks later the AD models were established. Diazoxide were  injected into some rats at the same time. Y maze electric stimulation was used to evalue the capability of study and memory of the rats, and protein electrophoresis was used to detect the expressions of Bcl-2 and Caspase-3 in cortical layer and hippocampus of the rats. Results   The capability of study and memory descended, the expression of Bcl-2 decreased and the expression of Caspase-3 increased in the rats injected with Aβ1-42 compared with the normal rats and thoses injected with NS (P<0.05). The capability of study and memory raised, the expression of Bcl-2 increased and the expression of Caspase-3 decreased in the rats injected with Aβ1-42 and diazoxide compared with the rats injected with Aβ1-42 alone(P<0.05). Conclusions  The AD models were established by the injection of Aβ1-42 into the bilateral ventricles of the rats. Diazoxide can improve the capability of study and memory, increase Bcl-2 expression and decrease Caspase-3 expression. It is likely that diazoxide could resist neurons apoptosis induced by Aβ1-42.

Key words: Alzheimer's disease; Diazoxide; Amyloid betaprotein; Caspase 3; Rats, Wistar