Abstract:

Objective  To investigate the changes of the gene expressive profile of the human ovarian cancer cell line (SKOV3) after transfection with PDCD4. Methods  The human ovarian cancer cell line SKOV3 was transfected with plasmids (pDsRed2-N1 and pDsRed2-N1-PDCD4) by using lipofectamine transfection reagent. SKOV3 cells stably expressing PDCD4 were established. Changes of the gene expression were analyzed by using gene chip technology. The related gene expression was detected by RT-PCR to confirm the results of the gene chip. Results  PDCD4 transfection induce widespread changes of the gene expressive profile of the human ovarian cancer cellline SKOV3. The chip data suggested that there were 467 differentially expressed genes, in which 255 genes were up-regulated and 212 genes were down-regulated. Five of the differentially expressed genes were detected by RT-PCR, which was consistent with the chip data. Conclusion  Gene chip technology was successfully used to screen the genes differentially expressed in the human ovarian cancer cell line SKOV3 transfected with PDCD4. This study provides laboratory evidence for further studying the tumor suppression mechanism of PDCD4.

Key words: PDCD4 genes； Ovarian tumor； Gene chip technology；Differentially expressed genes