Abstract:

To optimize an extracting process for the immunity active ingredient of Chinese  Herbal Medicine. MethodsThe extracting process was optimized by a uniform design with extraction rate, macrophage phagocytic function and lymphopoiesis rate as indexes to observe the effect of extraction temperature, amount of added extract solvent, extraction time and alcohol concentration on the immunity function of rats. ResultsThrough a DPS software analysis, the most favorable condition was with 20 times the amount of water as medicine, 100?℃, 170?min, and 85% of the alcohol concentration ratio. ConlusionThis extraction technique for the immunity active ingredient of Chinese Herbal Medicine is suitable and feasible.

Key words: Uniform design, Extracting process, Immunity active ingredient