JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2009, Vol. 47 ›› Issue (10): 1-4.
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CHEN Haiyan, LI Minglong, MA Lichuan, LIN Aiqing, LI Yinyin, ZHAO Jiajun
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Abstract:
To investigate the effects of a longterm isocaloric highprotein diet on morphology and function of the pancreatic islet in dietinduced obese rats. Methods: Dietinduced obese rat models were established by feeding a fatenriched diet. The obese male rats were randomly divided into three groups: the highprotein diet group (HP group, n=12), the highfat diet group (HF group, n=11), and the normal food group (NC group, n=11). The total calorie ingestion of each rat per day was similar and was maintained for 24 weeks. Then body weight, visceral fat mass and fasting plasma glucagonlike peptide1 (GLP1) were determined. The insulin secretory function of the islet was evaluated by an intravenous glucose tolerance test (IVGTT), and morphological analysis of the pancreatic islet was assessed by immunohistochemistry. ResultsCompared with the NC group, body weight [(490.92±39.47)g vs (545.55±31.08)g, P<0.01] and visceral fat mass [(22.42±7.04)g vs (32.33±9.27)g, P<0.05] in HP rats were significantly lower, but those indices were both higher [(656.01±58.49)g vs (545.55±31.08)g, (55.33±17.81)g vs(32.33±9.27)g, and both P<0.01] in the HF group. As to IVGTT, there were no differences in blood glucose among the three groups, but the serum insulin levels at 5min and 10 min were significantly lower in HP than in NC [(91.56±21.72) mIU/L vs (121.29±34.03) mIU/L; (58.62±15.80) mIU/L vs (81.12±24.36) mIU/L, both P<0.05], and 0 min and 10 min insulin levels were significantly higher in HF than in NC[(40.21±14.12) mIU/L vs (27.48±11.31) mIU/L,(98.15±27.58) mIU/L vs (81.12±24.36) mIU/L, both P<0.05]. Plasma GLP1 level was significantly lower in HP rats [(0.52±0.13)μg/L vs(0.71±0.19)μg/L,P<0.05], and there was an increased trend in the HF group [(1.03±0.28)μg/L vs(0.71±0.19)μg/L, P=0.11] compared with the NC group. Immunohistochemistry showed that there were no differences in morphology between HP and NC rats, but HF rats had a larger islet size,a lower ratio of insulin positive area to the islet,and a higher grey level(all P<0.05)compared with NC rats. ConclusionCompared with impairment of islet morphology and function by highfat diets,longterm isocaloric highprotein intake may reduce the first phase secretion of insulin in dietinduced obese rats, but there was no alteration in islet morphology. The decrease of insulin secretion may be related to GLP1 decline and weight loss.
Key words: Dietinduced obesity; Protein; Insulin; Immunohistoc hemistry; Glucagonlike peptide1; Rats, Wista
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