Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (10): 86-92.doi: 10.6040/j.issn.1671-7554.0.2019.344

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Effects of mifepristone and misoprostol on the expression of TRIM22 in trophoblast cells

ZHAO Limei1, YAN Lei2, SHEN Xiaochang1, SUN Yiqing1, HE Pengjuan1, ZHAO Xingbo1   

  1. 1. Department of Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    2. Department of Gynecology, Reproductive Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To investigate the effects of mifepristone and misoprostol on the expression of TRIM22 in trophoblast cells. Methods Forty cases of early pregnancy were divided into embryo aborting group(group A, n=12), artificial abortion group(group B, n=14)and drug abortion group(group C, n=14). HTR-8 cells were cultured in vitro and randomly divided into blank control group(group D), misoprostol group(group E), mifepristone group(group F), and mifepristone plus misoprostol group(group G)according to 2×2 factorial design. CCK-8 assay was used to detect the proliferation of cells, and cell scratch test was used to detect the migration ability of cells. The expression site and intensity of TRIM22 protein were detected by immunohistochemistry. Real-time fluorescence quantitative PCR and Western blotting were used to detect the expression of TRIM22 mRNA and protein and semi-quantitative analysis was performed. Results In the clinical cases, TRIM22 was mainly expressed in the nucleus of trophoblasts; the expression intensity of TRIM22 protein and mRNA in groups A and C was lower than that in group B(P<0.05), but there was no 山 东 大 学 学 报 (医 学 版)57卷10期 -赵立美,等.米非司酮、米索前列醇对滋养细胞TRIM22表达的影响 \=- significant difference between groups A and C (P>0.05). For the in vitro model of trophoblasts, the proliferation and migration ability of trophoblasts, and the expressions of TRIM22 protein and mRNA in the groups using mifepristone(group F, group G)were decreased compared with the groups without mifepristone(group D, group E)(P<0.001). There was no significant difference between misoprostol groups(group E, group G)and non-misoprostol group(group D, group F)(P>0.05); there was no significant interaction between mifepristone and misoprostol (P>0.05). Conclusion Mifepristone may inhibit the proliferation and migration of trophoblasts by down-regulating the expression of TRIM22. Misoprostol has no significant effect on the proliferation and migration of trophoblasts.

Key words: TRIM22, Mifepristone, Misoprostol, Trophoblast cells, Drug abortion

CLC Number: 

  • R714
[1] Versteeg GA, Rajsbaum R, Sánchezaparicio MT, et al. The E3-ligase TRIM family of proteins regulates signaling pathways triggered by innate immune pattern-recognition receptors[J]. Immunity, 2013, 38(2): 384-398.
[2] Rajsbaum R, García-Sastre A, Versteeg GA. TRIMmunity: the roles of the TRIM E3-ubiquitin ligase family in innate antiviral immunity[J]. J Mol Biol, 2014, 426(6): 1265-1284.
[3] Nautiyal J, Kumar PG, Laloraya M. Mifepristone(Ru486)antagonizes monocyte chemotactic protein-3 down-regulation at early mouse pregnancy revealing immunomodulatory events in Ru486 induced abortion[J]. Am J Reprod Immunol, 2015, 52(1): 8-18.
[4] Mao G, Wang J, Kang Y, et al. Progesterone increases systemic and local uterine proportions of CD4+CD25+ Treg cells during midterm pregnancy in mice[J]. Endocrinology, 2010, 151(11): 5477-5488.
[5] Cechin SR, Buchwald P. Effects of representative glucocorticoids on TNFα- and CD40L-induced NF-κB activation in sensor cells[J]. Steroids, 2014, 85(33): 36-43.
[6] Danielsson KG, Marions L, Bygdeman M. Effects of mifepristone on endometrial receptivity[J]. Steroids, 2003, 68(10): 1069-1075.
[7] 孙颖,郑晓静,李明江,等.米非司酮对早孕胎盘绒毛CEACAM6表达的影响[J].山东医药, 2013, 53(1): 43-44.
[8] 于鹏,颜磊,王飞,等.米非司酮对体外培养绒毛组织SPAG9表达的影响[J]. 医学与哲学(临床决策论坛版), 2011, 32(1): 50-51. YU Peng, YAN Lei, WANG Fei, et al. Effect of mifepristone on the expression of SPAG9 in villous explants[J]. Medicine and Philosophy(Clinical Decision Making Forum Edition), 2011, 32(1): 50-51.
[9] 鹿群,赵兴波,李建峰.米非司酮对人早孕绒毛、蜕膜组织血管生成的影响探讨[J].中国计划生育学杂志, 2008(2): 82-85. doi: 10.3969/j.issn.1004-8189.2008.02.008. LU Qun, ZHAO Xingbo, LI Jianfeng. Probe into the effect of mifepristone on angiogenesis of human chorionic villi and decidual cells[J]. Chinese Journal of Family Planning, 2008(2): 82-85. doi: 10.3969/j.issn.1004-8189.2008.02.008.
[10] 韩雪川,李明江,赵兴波,等.米非司酮对人早孕绒毛组织中TGFβ/smads信号通路功能状态的影响[J].山东大学学报(医学版), 2009, 47(7): 93-95. HAN Xuechuan, LI Mingjiang, ZHAO Xingbo, et al. Mifepristone modulates the function of TGF-β smads signaltransduction pathway in early pregnant chorionic villi[J]. Journal of Shandong University(Health Sciences), 2009, 47(7): 93-95.
[11] 郑晓静,孙颖,刘姝,等.米非司酮对人早孕绒毛组织核转录因子-κB表达的影响[J].山东大学学报(医学版), 2010, 48(1): 106-108. ZHENG Xiaojing, SUN Ying, LIU Shu, et al. Effect of mifepristone on expression of nuclear factor-κB in human villi during early pregnancy[J]. Journal of Shandong University(Health Sciences), 2010, 48(1): 106-108.
[12] Tocchini C, Ciosk R. TRIM-NHL proteins in development and disease[J]. Semin Cell Dev Biol, 2015(47-48): 52-59. doi: 10.1016/j.semcdb.2015.10.017.
[13] Uchil PD, Hinz A, Siegel S, et al. TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity[J]. J Virol, 2013, 87(1): 257-272.
[14] Wu X, Anderson JL, Campbell EM, et al. Proteasome inhibitors uncouple rhesus TRIM5alpha restriction of HIV-1 reverse transcription and infection[J]. Proc Natl Acad Sci U S A, 2006, 103(19): 7465-7470.
[15] Tocchini C, Keusch JJ, Miller SB, et al. The TRIM-NHL protein LIN-41 controls the onset of developmental plasticity in caenorhabditis elegans[J]. PLoS Genet, 2014, 10(8): e1004533. doi: 10.1371/journal.pgen.1004533.
[16] Li H, Sun L, Tang Z, et al. Overexpression of TRIM24 correlates with tumor progression in non-small cell lung cancer[J]. PLoS One, 2012, 7(5): e37657. doi: 10.1371/journal.pone.0037657.
[17] Zaman MU, Nomura T, Takagi T, et al. Ubiquitination-deubiquitination by the TRIM27-USP7 complex regulates tumor necrosis factor alpha-induced apoptosis[J]. Mol Cell Biol, 2013, 33(24): 4971-4984.
[18] Horie-Inoue K. TRIM proteins as trim tabs for the homoeostasis[J]. J Biochem, 2013, 154(4): 309-312.
[19] 王雨涛,周济,杨冬,等.TRIM29基因在胶质瘤中的表达及其对胶质母细胞瘤细胞U-87 MG增殖与迁移的影响[J]. 第三军医大学学报, 2018, 40(11): 959-965. WANG Yutao, ZHOU Ji,YANG Dong, et al. Expression of TRIM29 in gliomas and its effect on proliferation and migration of glioblastoma U-87 MG cells[J]. Journal of Third Military Medical University 2018, 40(11): 959-965.
[20] 龙璐,王堃,陈贞,等.三结构域包含蛋白29对鼻咽癌细胞生长和迁移的影响[J].中国现代医学杂志, 2015, 25(2): 7-12. LONG Lu, WANG Kun, CHEN Zhen, et al. Effects of TRIM 29 on growth and migration of nasopharyngeal carcinoma cells[J]. China Journal of Modern Medicine. 2015, 25(2): 7-12.
[21] Zhou XM, Sun R, Luo DH, et al. Upregulated TRIM29 promotes proliferation and metastasis of nasopharyngeal carcinoma via PTEN/AKT/mTOR signal pathway[J]. Oncotarget, 2016, 7(12): 13634-13650.
[22] Murray MJ, Lessey BA. Embryo implantation and tumor metastasis: common pathways of invasion and angiogenesis[J]. Semin Reprod Endocrinol, 1999, 17(3): 275-290.
[23] 高波,段志坚,徐薇,等.抗病毒固有免疫分子TRIM22 C端SPRY结构域对其转录、翻译及亚细胞定位的影响[J].微生物与感染, 2010, 5(1): 31-35. GAO Bo, DUAN Zhijian, XU Wei, et al. The contribution of the C terminal SPRY domain of TRIM22 to its own transcription, translation, and subcellular localization[J]. Journal of Microbes and Infection, 2010, 5(1): 31-35.
[24] Saito-Kanatani M, Urano T, Hiroi H, et al. Identification of TRIM22 as a progesterone-responsive gene in Ishikawa endometrial cancer cells[J]. J Steroid Biochem Mol Biol, 2015, 154: 217-225. doi: 10.1016/j.jsbmb.2015.08.024.
[25] Obad S, Brunnström H, Vallonchristersson J, et al. Staf50 is a novel p53 target gene conferring reduced clonogenic growth of leukemic U-937 cells[J]. Oncogene, 2004, 23(23): 4050.
[26] Sun Y, Ho GH, Koong HN, et al. Down-regulation of Tripartite-motif containing 22 expression in breast cancer is associated with a lack of p53-mediated induction[J]. Biochem Biophys Res Commun, 2013, 441(3): 600-606.
[27] Zirn B, Hartmann O, Samans B, et al. Expression profiling of Wilms tumors reveals new candidate genes for different clinical parameters[J]. Int J Cancer, 2010, 118(8): 1954-1962.
[28] Vicent GP, Zaurin R, Nacht AS, et al. Nuclear factor 1 synergizes with progesterone receptor on the mouse mammary tumor virus promoter wrapped around a histone H3/H4 tetramer by facilitating access to the central hormone-responsive elements[J]. J Biol Chem, 2010, 285(4): 2622-2631.
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