JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (2): 55-60.doi: 10.6040/j.issn.1671-7554.0.2016.288

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Effects of different doses of perindopril on endothelial progenitor cells and systolic cardiac function in rabbits with acute myocardial infarction

TAN Hong1, MENG Nan1, JIN Qun1, SU Li2, ZHANG Xiaxiao1, CHEN Yingjian3, HAO Zhe1, LIU Xiaohong4   

  1. 1. Department of Cardiology;
    2. 2nd Department of Health;
    3. Department of Clinical Laboratory;
    4. Department of Pathology, General Hospital of Jinan Military Command, Jinan 250031, Shandong, China
  • Received:2016-03-17 Online:2017-02-10 Published:2017-02-10

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Abstract: Objective To investigate the effects of different doses of perindopril on mobilization of endothelial progenitor cells(EPCs)and the impact on cardiac systolic function in rabbits with acute myocardial infarction(AMI). Methods Rabbit models of acute myocardial infarction were established and randomly divided into 4 groups: sham-operated group(n=7), AMI group(n=6), low dose perindopril group(n=6, 0.218 mg/kg of perindopril was given a day after the model of AMI was built), high dose perindopril group(n=6, 0.436 mg/kg of perindopril was given a day after the model of AMI was built). The number of EPCs in circulating blood on day 1, 3, 7, 14, and 28 was counted with flow cytometry. On day 28, the stroke volume(SV), left ventricular ejection fraction(LVEF)and left ventricular fractional shortening(LVFS)were examined with cardiac Doppler ultrasound. The necrosis and disarrangement 山 东 大 学 学 报 (医 学 版)55卷2期 -谈红,等.不同剂量培哚普利对兔急性心肌梗死后内皮祖细胞及心功能的影响 \=-of cardiomyocytes in each group was observed after HE staining of infarcted cardiac areas. Results After perindopril treatment, there were significant increases of EPCs level in AMI rabbits, especially in high perindopril dose group(P<0.05). On day 28, there were significant decreases of LVFS, SV and LVEF in AMI group compared with sham-operated group(P<0.05). LVFS, SV and LVEF significantly increased after perindopril treatment, especially in high perindopril dose group(P<0.05). There was correlation between EPCs level and LVEF in all groups(R=0.468, P=0.05). HE staining showed that the normal cardiomyocytes in AMI group were replaced by large numbers of scar tissues with lymphocyte infiltration. Small quantity of pycnosis cardiomyocytes and relatively more scar tissues were seen in low dose perindopril group, and the degree of necrosis and disarrangement was lower than that in AMI group. The changes were more obvious in high dose perindopril group, in which more normal cardiomyocytes and fewer scar tissues were observed. Conclusion Perindopril can promote the mobilization of EPCs, elevate LVEF, LVFS and SV, and improve cardiac systolic function in AMI rabbits. High dose of perindopril is more efficient. There is correlation between EPCs level and the improvement of cardiac systolic function.

Key words: Perindopril, Endothelial progenitor cells, Cardiac systolic function, Acute myocardial infarction, Degree of cardiomyocyte disarrangement

CLC Number: 

  • R541.4
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