Abstract:

Objective  To investigate the effect of human umbilical cord mesenchymal stem cells (HUC-MSCs) transplantation combined with hematoma aspiration in the therapy of rats with intracerebral hemorrhage by observing the changes of p53 gene expression and the recovery of neural functions. Methods  HUC-MSCs were isolated in vitro and labeled by BrdU before transplantation. 2μL Ⅳ collagenase was injected to the caudate nucleus to establish intracerebral hemorrhage model. One hundred and twenty SD rats with successful modeling were randomly divided into 4 groups: intracerebral hemorrhage group, hematoma aspiration group, HUC-MSCs transplantation group and HUC-MSCs transplantation combined with hematoma aspiration group(combination group). After the corresponding treatments, rats in each group were killed on 5 time points(days 1, 3, 7, 14 and 30), then the brain tissues were isolated. The expressions of p53 in the cerebral tissues surrounding intracerebral hemorrhage on different time points and the distribution of HUC-MSCs were detected by immunohistochemical staining. Neural functional status of rats was assessed by modified neurological severity score(mNSS). Results  The mNSS scores and the expression of p53 in hematoma aspiration group, HUC-MSCs transplantation group and combination group were significantly lower than those in intracerebral hemorrhage group (all P<0.05). The scores of mNSS and the expression of p53 in combination group were lower than those in intracerebral hemorrhage group and hematoma aspiration group on day 7 (all P<0.05), and lower than those in the other three groups from day 14 to day 30. Conclusion  The intervention of HUC-MSCs transplantation combined with hematoma aspiration can obviously reduce the damage of nerve cells in rats with intracerebral hemorrhage and it is beneficial to the recovery of neural function.

Key words: Human umbilical cord mesenchymal stem cells; Hematoma aspiration; p53 gene; Intracerebral hemorrhage; Rats