Abstract:

Objective   To confirm the effect of mesenchymal stem cell (MSCs) on the growth of cervical carcinoma Hela cell line and elucidate the mechanism. Methods   MSCs were isolated from human umbilical cord. MSCs were cultured with Hela and the growth inhibitory effect of MSCs on cervical carcinoma was tested through Cell Counting Kit-8. The inhibitory effect of MSCs conditional culture media on Hela was examined using CCK-8 and colony formation assay. The apoptosis induction effect of MSCs condition culture media on Hela was assessed with RT-PCR assay. Results   Human umbilical cord MSCs were successfully cultivated. CCK-8 showed that MSCs and its conditional culture media exhibited a number-dependent growth inhibitory effect on Hela cell lines in vitro. The number of colonies formed by Hela cultured with MSCs conditional culture media was significantly lower compared with the control group. RT-PCR showed that MSCs conditional culture media could upregulate proapoptosis mRNA gene expression of Bax, P53 and Caspase-3, and inhibit anti-apoptosis gene expression of Bcl-2 and survivin. Conclusion   MSCs could inhibit tumor cell growth in vitro by inducing cervical cancer cell apoptosis.

Key words: Mesenchymal stem cells; Tumor; Proliferation; Apoptosis