Abstract:

Objective   To study the signal pathway of endothelial lipase (EL) expression regulated by angiotensin Ⅱ (AngⅡ). Methods   The human umbilical vein endothelial cells (HUVECs) were cultured in vitro and divided into 3 groups. ①The AngⅡ group (10umol/L AngⅡ was added); ②The PDTC group(pretreated by 10mmol/L PDTC); ③The control group(no stimulating factors was added). After incubation of 2、4、8、12、24h respectively, the HUVECs were collected and then EL and NF-κB p65 in HUVECs were detected by Western blot. Results   AngⅡ increased the expression of EL and NF-κB p65 in HUVECs, and two proteins rose in the same trend. EL expressions at 4、8、12h were significantly higher than those of the control group(P<0.05) and NF-κB p65 expressions at 2、4、8、12h were significantly higher than those of the control group(P<0.05) after the stimulating of AngⅡ. PDTC down regulated the expression of EL After treated with PDTC, the expressions of EL decreaseed significantly at 2、4、8、12、24h than those of the AngⅡ group at the same time (P<0.05). Conclusion   AngⅡ may increace the expression of EL through the signal pathway of NF-κB p65.

Key words: Human umbilical vein endothelial cells； Endothelial lipase； Angiotension Ⅱ; NF-κB p65